Ghosh Papita, Muthuraj Thamil Selvan, Bandyopadhyay Prasanta, Swarnakar Snehasikta, Sarkar Puja, Varatharajan Abinaya
Department of Periodontics, Dr R Ahmed Dental College and Hospital, Jadavpur, Kolkata, India.
Department of Periodontics, CSI College of Dental Sciences and Research, Madurai, Tamil Nadu, India.
J Indian Soc Periodontol. 2021 Sep-Oct;25(5):386-392. doi: 10.4103/jisp.jisp_811_20. Epub 2021 Aug 30.
Matrix metalloproteinases (MMPs) are a group of host-derived zinc-dependent enzymes which mediates the destruction of the extracellular matrix. In periodontitis, there is excess production of MMPs associated with periodontal tissue destruction. The aim of this study was to estimate the level MMP-9 in both active and latent form in gingival tissue (GT) samples collected from periodontitis patients with different rates of progression and compare it with healthy individuals.
Sixty patients were selected and divided into three groups, 20 each: Group A (slowly/moderately progressing periodontitis), Group B (rapidly progressing periodontitis), and Group C (clinical periodontal health). Plaque index, gingival index, periodontal probing depth (PPD), and clinical attachment level were recorded. GT samples were collected from all 60 patients and MMP-9 expressions were measured using gelatin zymography and western blotting.
Levels of active MMP-9 (aMMP-9) and latent MMP-9 (lMMP-9) were significantly high in both Group A (GA) (aMMP-9: 2.05 arbitrary unit [AU]/lMMP-9: 2.54 AU) and Group B (GB) (aMMP-9: 1.32 AU/lMMP-9: 1.74 AU) when compared to that of Group C (GC) (aMMP-9: 0.93/lMMP-9: 1.08 AU). In GA, levels of aMMP-9 showed a significant correlation with PPD values. No other correlations were found.
The levels of aMMP-9 and lMMP-9 were increased in both the types of periodontitis when compared with periodontally healthy individuals. A significant correlation was found between PPD and activities of aMMP-9 in slowly/moderately progressing periodontitis patients. However, further studies are required to confirm these findings.
基质金属蛋白酶（MMPs）是一组宿主来源的锌依赖性酶，可介导细胞外基质的破坏。在牙周炎中，MMPs的过量产生与牙周组织破坏有关。本研究的目的是评估从不同进展速率的牙周炎患者收集的牙龈组织（GT）样本中活性和潜伏形式的MMP-9水平，并将其与健康个体进行比较。
选择60例患者并分为三组，每组20例：A组（缓慢/中度进展性牙周炎）、B组（快速进展性牙周炎）和C组（临床牙周健康）。记录菌斑指数、牙龈指数、牙周探诊深度（PPD）和临床附着水平。从所有60例患者中收集GT样本，并使用明胶酶谱法和蛋白质印迹法测量MMP-9表达。
与C组（GC）（活性MMP-9：0.93/潜伏MMP-9：1.08 AU）相比，A组（GA）（活性MMP-9：2.05任意单位[AU]/潜伏MMP-9：2.54 AU）和B组（GB）（活性MMP-9：1.32 AU/潜伏MMP-9：1.74 AU）的活性MMP-9（aMMP-9）和潜伏MMP-9（lMMP-9）水平均显著升高。在GA组中，aMMP-9水平与PPD值呈显著相关性。未发现其他相关性。
与牙周健康个体相比，两种类型的牙周炎中aMMP-9和lMMP-9水平均升高。在缓慢/中度进展性牙周炎患者中，PPD与aMMP-9活性之间存在显著相关性。然而，需要进一步研究来证实这些发现。
