Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, People's Republic of China.
Curr Microbiol. 2021 Dec;78(12):4115-4126. doi: 10.1007/s00284-021-02671-x. Epub 2021 Oct 20.
Grey mould caused by Botrytis cinerea leads to severe economic loss on commercial tomato production. Application of beneficial microorganism offers an eco-friendly alternative for mitigation of tomato fungal disease damage, considering negative influences of fungicides. In the present study, an antagonistic Trichoderma afroharzianum isolate TM24 was evaluated for its biocontrol potential on tomato grey mould. The isolate TM24 showed obviously antagonistic effect on B. cinerea mycelium growth and production of glucanase and chitinase. Leaf spraying with spore suspension of isolate TM24 showed a biocontrol efficiency of over 54% against tomato grey mould in greenhouse pot experiment. The activities of plant defense-related enzymes including polyphenol oxidase, phenylalanine ammonialyase, superoxide dismutase, and peroxidase were all increased to varying degrees in tomato leaves after isolate TM24 treatment. Transcriptome analysis showed that, a total of 1941, 1753 and 38 differentially expressed genes (DEGs) were obtained at 24, 48 and 72 hpi, respectively, in tomato leaves pretreated with T. afroharzianum TM24, and then challenged with B. cinerea inoculation. The DEGs were mainly enriched in MAPK signaling pathway and plant hormones signal transduction pathway. Multiple genes that regulated crucial nodes of defense-related pathways, like flavonoid, phenylpropanoid, jasmonic acid and ethylene metabolisms were also identified, which may have positive correlations with the biocontrol potential of isolate TM24 in tomato plants. These promising results provided valuable information on using T. afroharzianum TM24 as a beneficial biocontrol agent in tomato grey mould management.
灰霉病是由灰葡萄孢引起的，它会导致商业番茄生产遭受严重的经济损失。考虑到杀菌剂的负面影响，有益微生物的应用为减轻番茄真菌病害提供了一种环保的替代方案。在本研究中，评估了一种拮抗 Trichoderma afroharzianum 分离株 TM24 对番茄灰霉病的生物防治潜力。该分离株 TM24 对 B. cinerea 菌丝生长和葡聚糖酶和几丁质酶的产生表现出明显的拮抗作用。温室盆栽试验中，用分离株 TM24 的孢子悬浮液喷洒叶片，对番茄灰霉病的防治效果超过 54%。在分离株 TM24 处理后，番茄叶片中植物防御相关酶的活性，如多酚氧化酶、苯丙氨酸解氨酶、超氧化物歧化酶和过氧化物酶，均有不同程度的增加。转录组分析表明，在番茄叶片用 T. afroharzianum TM24 预处理后，分别在 24、48 和 72 hpi 时，共获得了 1941、1753 和 38 个差异表达基因（DEGs），然后用 B. cinerea 接种。DEGs 主要富集在 MAPK 信号通路和植物激素信号转导通路。还鉴定了多个调节防御相关途径关键节点的基因，如类黄酮、苯丙烷类、茉莉酸和乙烯代谢，这可能与分离株 TM24 在番茄植物中的生物防治潜力有正相关关系。这些有希望的结果为利用 T. afroharzianum TM24 作为番茄灰霉病管理中的有益生物防治剂提供了有价值的信息。
