Determination of Reactive Oxygen or Nitrogen Species and Novel Volatile Organic Compounds in the Defense Responses of Tomato Plants against Induced by TRS 106.

In the present study, TRS 106 decreased grey mould disease caused by in tomato plants ( L.) by enhancing their defense responses. Generally, plants belonging to the 'Remiz' variety, which were infected more effectively by than 'Perkoz' plants, generated more reactive molecules such as superoxide (O) and peroxynitrite (ONOO), and less hydrogen peroxide (HO), S-nitrosothiols (SNO), and green leaf volatiles (GLV). Among the new findings, histochemical analyses revealed that infection caused nitric oxide (NO) accumulation in chloroplasts, which was not detected in plants treated with TRS 106, while treatment of plants with TRS 106 caused systemic spreading of HO and NO accumulation in apoplast and nuclei. SPME-GCxGC TOF-MS analysis revealed 24 volatile organic compounds (VOC) released by tomato plants treated with TRS 106. Some of the hexanol derivatives, e.g., 4-ethyl-2-hexynal and 1,5-hexadien-3-ol, and salicylic acid derivatives, e.g., 4-hepten-2-yl and isoamyl salicylates, are considered in the protection of tomato plants against for the first time. The results are valuable for further studies aiming to further determine the location and function of NO in plants treated with and check the contribution of detected VOC in plant protection against .