Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
Institute of Natural Products and Cosmetics, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, Poland.
Cells. 2022 Sep 29;11(19):3051. doi: 10.3390/cells11193051.
In the present study, TRS 106 decreased grey mould disease caused by in tomato plants ( L.) by enhancing their defense responses. Generally, plants belonging to the 'Remiz' variety, which were infected more effectively by than 'Perkoz' plants, generated more reactive molecules such as superoxide (O) and peroxynitrite (ONOO), and less hydrogen peroxide (HO), S-nitrosothiols (SNO), and green leaf volatiles (GLV). Among the new findings, histochemical analyses revealed that infection caused nitric oxide (NO) accumulation in chloroplasts, which was not detected in plants treated with TRS 106, while treatment of plants with TRS 106 caused systemic spreading of HO and NO accumulation in apoplast and nuclei. SPME-GCxGC TOF-MS analysis revealed 24 volatile organic compounds (VOC) released by tomato plants treated with TRS 106. Some of the hexanol derivatives, e.g., 4-ethyl-2-hexynal and 1,5-hexadien-3-ol, and salicylic acid derivatives, e.g., 4-hepten-2-yl and isoamyl salicylates, are considered in the protection of tomato plants against for the first time. The results are valuable for further studies aiming to further determine the location and function of NO in plants treated with and check the contribution of detected VOC in plant protection against .
在本研究中，TRS 106 通过增强防御反应来降低番茄植株（L.）上由引起的灰霉病。一般来说，与‘Perkoz’植株相比，更容易被感染的‘Remiz’品种的植物会产生更多的活性分子，如超氧阴离子（O）和过氧亚硝酸盐（ONOO），而产生的过氧化氢（HO）、S-亚硝基硫醇（SNO）和绿叶挥发物（GLV）较少。在新的发现中，组织化学分析表明，感染导致叶绿体中一氧化氮（NO）积累，而用 TRS 106 处理的植物中未检测到，而用 TRS 106 处理的植物则导致 HO 和 NO 在质外体和核中系统扩散和积累。SPME-GCxGC TOF-MS 分析显示，用 TRS 106 处理的番茄植株释放出 24 种挥发性有机化合物（VOC）。一些己醇衍生物，如 4-乙基-2-己炔醛和 1,5-己二烯-3-醇，以及水杨酸衍生物，如 4-庚烯-2-基和异戊基水杨酸酯，在保护番茄植物免受首次被认为是针对的。这些结果对于进一步研究具有重要价值，旨在进一步确定用和检测到的 VOC 在植物保护中的作用，以防止。
