Valença I N, Rós F A, Zucherato V S, Silva-Pinto A C, Covas D T, Kashima S, Slavov S N
Programa de Pós-Graduação em Oncologia Clínica, Células Tronco e Terapia Celular, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Hemocentro de Ribeirão Preto, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Braz J Med Biol Res. 2021 Oct 18;54(12):e11610. doi: 10.1590/1414-431X2021e11610. eCollection 2021.
Due to the high transfusion volume, polytransfused patients with sickle cell disease (SCD) and beta-thalassemia are constantly exposed to parenterally transmitted infections. Currently, we have little information about the virome of such patients and how the virological composition might be influenced by the hemotherapy procedures that these patients receive. The objective of this study was to compare the viral diversity between these two groups with respect to the viral abundance and how it might be affected by the specific conditions of these groups. We sequenced by next-generation sequencing (NGS) and compared the virome of 30 patients with beta-thalassemia major, 45 with SCD, and 16 blood donors from the Blood Center of Ribeirão Preto, Brazil. Predominantly, commensal viruses including Torque teno virus (TTV) genotypes and human pegiviris-1 (HPgV-1) were identified in each group. Strikingly, while HPgV-1 reads were dominant in the SCD group, thalassemic patients showed high TTV abundance, expressed both in viral reads and genotypes. We speculated that the commensal virome of polytransfused patients might be influenced by the transfusion frequency and disease characteristics and that commensal viruses might be used as important genetic biomarkers for these hematological disturbances. Nevertheless, more specific studies are necessary to confirm a relationship between blood virome and transfusion treatment.
由于输血量大，镰状细胞病（SCD）和β地中海贫血的多次输血患者持续面临经肠道外传播的感染风险。目前，我们对这类患者的病毒组以及这些患者接受的血液疗法如何影响病毒学组成了解甚少。本研究的目的是比较这两组患者在病毒丰度方面的病毒多样性，以及病毒多样性如何受到这些组的特定条件的影响。我们通过下一代测序（NGS）进行测序，并比较了30例重型β地中海贫血患者、45例SCD患者以及巴西里贝朗普雷图血液中心的16名献血者的病毒组。主要在每组中鉴定出包括细小病毒B19基因型和人pegivirus-1（HPgV-1）在内的共生病毒。令人惊讶的是，虽然HPgV-1读数在SCD组中占主导地位，但地中海贫血患者显示出高细小病毒B19丰度，在病毒读数和基因型中均有表达。我们推测，多次输血患者的共生病毒组可能受到输血频率和疾病特征的影响，并且共生病毒可能用作这些血液系统紊乱的重要遗传生物标志物。然而，需要更具体的研究来证实血液病毒组与输血治疗之间的关系。
