Luciola Zanette Dalila, Andrade Coelho Karoline Brito Caetano, de Carvalho Eneas, Aoki Mateus Nobrega, Nardin Jeanine Marie, Araújo Lalli Larissa, Dos Santos Bezerra Rafael, Giovanetti Marta, Simionatto Zucherato Victória, Montenegro de Campos Gabriel, de Souza Todão Bernardino Jardelina, Louis Viala Vincent, Ciccozzi Massimo, Junior Alcantara Luiz Carlos, Coccuzzo Sampaio Sandra, Elias Maria Carolina, Kashima Simone, Tadeu Covas Dimas, Nanev Slavov Svetoslav
Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ), Professor Algacyr Munhoz Mader, Curitiba, Parana, Brazil.
Department of Anatomy and Surgery, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirao Preto, Sao Paulo, Brazil.
Mol Cell Oncol. 2023 Mar 15;10(1):2188858. doi: 10.1080/23723556.2023.2188858. eCollection 2023.
Growing evidence suggests that metavirome changes could be associated increased risk for malignant cell transformation. Considering Viruses have been proposed as factors for prostate cancer induction. The objective of this study was to examine the composition of the plasma metavirome of patients with prostate cancer. Blood samples were obtained from 49 male patients with primary prostate adenocarcinoma. Thirty blood donors were included as a control group. The obtained next-generation sequencing data were analyzed using a bioinformatic pipeline for virus metagenomics. Viral reads with higher abundance were assembled in contigs and analyzed taxonomically. Viral agents of interest were also confirmed by qPCR. Anelloviruses and the Human Pegivirus-1 (HPgV-1) were the most abundant component of plasma metavirome. Clinically important viruses like hepatitis C virus (HCV), cytomegalovirus and human adenovirus type C were also identified. In comparison, the blood donor virome was exclusively composed of torque teno virus types (TTV) types. The performed HPgV-1 and HCV phylogeny revealed that these viruses belong to commonly detected in Brazil genotypes. Our study sheds light on the plasma viral abundance in patients with prostatic cancer. The obtained viral diversity allowed us to separate the patients and controls, probably suggesting that malignant processes may influence virome composition. More complex and multiple approach investigations are necessary to examine the likely causal relationship between metavirome and its nvolvement in prostate cancer.
越来越多的证据表明，病毒宏基因组的变化可能与恶性细胞转化风险增加有关。鉴于病毒已被认为是前列腺癌诱发因素。本研究的目的是检测前列腺癌患者血浆病毒宏基因组的组成。从49例原发性前列腺腺癌男性患者中采集血样。纳入30名献血者作为对照组。使用病毒宏基因组学的生物信息学流程分析获得的二代测序数据。丰度较高的病毒读数组装成重叠群并进行分类分析。感兴趣的病毒病原体也通过定量聚合酶链反应进行确认。环曲病毒和人pegivirus-1（HPgV-1）是血浆病毒宏基因组中最丰富的成分。还鉴定出了丙型肝炎病毒（HCV）、巨细胞病毒和C型人腺病毒等具有临床重要性的病毒。相比之下，献血者的病毒组仅由细小病毒属（TTV）类型组成。对HPgV-1和HCV进行的系统发育分析表明，这些病毒属于在巴西常见的基因型。我们的研究揭示了前列腺癌患者血浆中的病毒丰度情况。所获得的病毒多样性使我们能够区分患者和对照组，这可能表明恶性过程可能影响病毒组的组成。需要更复杂和多样的研究方法来检验病毒宏基因组与其在前列腺癌中的作用之间可能存在的因果关系。
