Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Penn-CHOP Lung Biology Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Penn-CHOP Lung Biology Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Trends Mol Med. 2021 Dec;27(12):1159-1174. doi: 10.1016/j.molmed.2021.09.008. Epub 2021 Oct 18.
Organoids can be derived from various cell types in the lung, and they provide a reproducible and tractable model for understanding the complex signals driving cell fate decisions in a regenerative context. In this review, we provide a retrospective account of organoid methodologies and outline new opportunities for optimizing these methods to further explore emerging concepts in lung biology. Moreover, we examine the benefits of integrating organoid assays with in vivo modeling to explore how the various niches and compartments in the respiratory system respond to both acute and chronic lung disease. The strategic implementation and improvement of organoid techniques will provide exciting new opportunities to understand and identify new therapeutic approaches to ameliorate lung disease states.
类器官可以从肺部的各种细胞类型中衍生而来，为理解再生背景下驱动细胞命运决定的复杂信号提供了一种可重现和易于处理的模型。在这篇综述中，我们回顾了类器官方法，并概述了优化这些方法的新机会，以进一步探索肺部生物学中的新兴概念。此外，我们还研究了将类器官测定与体内建模相结合的优势，以探索呼吸系统的各种小生境和隔室如何对急性和慢性肺部疾病做出反应。类器官技术的战略实施和改进将为理解和确定改善肺部疾病状态的新治疗方法提供令人兴奋的新机会。
