Laboratory of Pesticide Science, Department of Crop Science, Agricultural University of Athens, 75 Iera Odos Street, 11855, Athens, Greece.
Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium.
Exp Appl Acarol. 2021 Dec;85(2-4):205-221. doi: 10.1007/s10493-021-00665-9. Epub 2021 Oct 21.
The honey bee ectoparasite Varroa destructor is considered the major threat to apiculture, as untreated colonies of Apis mellifera usually collapse within a few years. In order to control this mite, many beekeepers rely on a limited number of approved synthetic acaricides, including the pyrethroids tau-fluvalinate and flumethrin. Due to the intensive use of these products, resistance is now commonplace in many beekeeping regions across the world. In the present study, the occurrence of amino acid substitutions at residue L925 of the voltage-gate sodium channel-the pyrethroid target site-was studied in Varroa populations collected throughout Flanders, Belgium. Dose-response bioassays supported the involvement of the frequently observed L925V substitution in flumethrin resistance, resulting in a 12.64-fold increase of the LC in a Varroa population mostly consisting of homozygous 925 V/V mites. With the presence of L925 substitutions in about four out of 10 screened apiaries, the use of pyrethroid-based varroacides in Flanders, including the recently released PolyVar® Yellow, should be carefully considered.
西方蜜蜂体外寄生虫瓦螨（Varroa destructor）被认为是养蜂业的主要威胁，因为未经处理的西方蜜蜂群体通常在几年内就会崩溃。为了控制这种螨虫，许多养蜂人依赖少数几种经过批准的合成杀螨剂，包括拟除虫菊酯tau-氟戊菊酯和氟氯氰菊酯。由于这些产品的大量使用，现在在世界许多养蜂地区，抗性已经很普遍。在本研究中，研究了在比利时佛兰德斯采集的瓦螨种群中电压门控钠离子通道（拟除虫菊酯靶标）残基 L925 处氨基酸取代的发生情况。剂量反应生物测定支持经常观察到的 L925V 取代与氟氯氰菊酯抗性有关，导致在主要由纯合 925V/V 螨虫组成的瓦螨种群中 LC 增加了 12.64 倍。在大约十分之四的筛查蜂场中存在 L925 取代，因此在佛兰德斯使用基于拟除虫菊酯的杀螨剂，包括最近发布的 PolyVar® Yellow，应该谨慎考虑。
