Apinov, Scientific Beekeeping and Training Centre, Lagord, France.
French Agency for Food, Environmental and Occupational Health and Safety (ANSES), Sophia Antipolis Laboratory, Bee Pathology Unit, Sophia Antipolis, France.
Pest Manag Sci. 2022 Dec;78(12):5097-5105. doi: 10.1002/ps.7126. Epub 2022 Sep 14.
Varroa destructor is a parasitic mite of the honey bee, Apis mellifera. Its presence in colonies can lead to a collapse within a few years. The use of acaricides has become essential to manage the hive infestation. However, the repeated and possibly incorrect use of acaricide treatments, as tau-fluvalinate, has led to the development of resistance. The in vitro phenotypic test allows the proportion of susceptible or resistant individuals to be known following an exposure to an active substance. In Varroa mites, resistance to tau-fluvalinate is associated with the presence of mutations at the position 925 of the voltage-gated sodium channel (VGSC).
Here, we compared the results obtained with an in vitro phenotypic test against tau-fluvalinate and those obtained with an allelic discrimination assay on 13 treated and untreated Varroa populations in France. The correlation between the phenotype and the genetic profile rate is found to be 0.89 Varroa mites having resistant phenotypic profile have a probability of 63% to present the L925V mutation (resistance detection reliability). However, 97% of the Varroa mites having the susceptible phenotype do not present the L925V mutation (susceptible detection reliability).
The L925V mutation explains most of the resistance to tau-fluvalinate in V. destructor in the populations tested. However, other mutations or types of resistance may also be involved to explain the survival of Varroa mites in the phenotypic test. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
瓦螨是蜜蜂，即 Apis mellifera 的寄生螨。其在蜂群中的存在可能导致在短短几年内蜂群崩溃。杀螨剂的使用已成为管理蜂群感染的必要手段。然而，由于重复使用和可能不正确使用杀螨剂（如 tau-氟戊菊酯），已经导致了抗药性的产生。体外表型测试可在接触活性物质后，知道对敏感或抗性个体的比例。在瓦螨中，对 tau-氟戊菊酯的抗性与电压门控钠离子通道（VGSC）位置 925 的突变有关。
在这里，我们比较了在法国对 13 个经处理和未经处理的瓦螨种群进行 tau-氟戊菊酯体外表型测试和等位基因鉴别检测的结果。表型和遗传特征率之间的相关性发现为 0.89，具有抗性表型特征的瓦螨有 63%的概率出现 L925V 突变（抗性检测可靠性）。然而，97%具有敏感表型的瓦螨不出现 L925V 突变（敏感检测可靠性）。
在所测试的种群中，L925V 突变解释了 tau-氟戊菊酯对瓦螨的大部分抗性。然而，其他突变或类型的抗性也可能涉及，以解释瓦螨在表型测试中的存活。© 2022 作者。害虫管理科学由 John Wiley & Sons Ltd 代表化学工业协会出版。
