Sun Hua, Zhao Ling, Xu Zi-Jian, De Marco Michele, Briens Mickael, Yan Xiang-Hua, Sun Lv-Hui
Hubei Hongshan Laboratory, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Adisseo France S.A.S., 10, Place du Général de Gaulle, 92160 Antony, France.
Antioxidants (Basel). 2021 Sep 28;10(10):1542. doi: 10.3390/antiox10101542.
This study has determined whether hydroxy-selenomethionine (OH-SeMet) exerts a better protective action on broilers against environmental stress than sodium selenite (SS) or seleno-yeast (SY). Day-old male Cobb 500 broilers (12 cages/diet, 9 broilers/cage) were fed a selenium (Se)-deficient diet (0.047 mg/kg) supplemented with SS, SY or OH-SeMet at 0.3 mg Se/kg under a high stocking density and heat stress condition for six weeks. OH-SeMet improved the FCR and Se concentration in the tissues than SS and SY. SY and OH-SeMet both reduced the serum cortisol, T3, IL-6, IgA, IgM and LPS, more than SS, while only OH-SeMet further increased IL-10 and IgG. SY and OH-SeMet improved the intestinal morphology and increased the T-AOC, TXRND, SELENON and OCCLUDIN activities but decreased CLAUDIN2 in the jejunum than SS, while OH-SeMet further improved these values than SY. SY and OH-SeMet both increased SELENOS and TXNRD2 in the muscles than SS, and OH-SeMet further raised T-AOC, GPX4, SELENOP, SELENOW and TXNRD1, and reduced malondialdehyde and protein carbonyl in the muscles than SS and SY. OH-SeMet showed a better ability to maintain the performance and the redox and immune status of broilers under a high stocking density and heat stress challenge than SS and SY.
本研究确定了羟基硒代蛋氨酸（OH-SeMet）对肉鸡抵抗环境应激的保护作用是否优于亚硒酸钠（SS）或硒酵母（SY）。将1日龄雄性科宝500肉鸡（每种日粮12个笼子，每个笼子9只肉鸡）饲养在高饲养密度和热应激条件下六周，饲喂缺硒日粮（0.047毫克/千克），并添加0.3毫克硒/千克的SS、SY或OH-SeMet。与SS和SY相比，OH-SeMet改善了饲料转化率和组织中的硒浓度。SY和OH-SeMet均比SS更能降低血清皮质醇、T3、IL-6、IgA、IgM和LPS，而只有OH-SeMet进一步提高了IL-10和IgG。与SS相比，SY和OH-SeMet改善了肠道形态，增加了空肠中的总抗氧化能力（T-AOC）、硫氧还蛋白还原酶（TXRND）、硒蛋白N（SELENON）和闭合蛋白（OCCLUDIN）活性，但降低了闭合蛋白2（CLAUDIN2），而OH-SeMet比SY进一步改善了这些值。与SS相比，SY和OH-SeMet均增加了肌肉中的硒蛋白S（SELENOS）和硫氧还蛋白还原酶2（TXNRD2），并且OH-SeMet比SS和SY进一步提高了肌肉中的T-AOC、谷胱甘肽过氧化物酶4（GPX4）、硒蛋白P（SELENOP）、硒蛋白W（SELENOW）和硫氧还蛋白还原酶1（TXNRD1），并降低了丙二醛和蛋白质羰基含量。在高饲养密度和热应激挑战下，OH-SeMet在维持肉鸡性能、氧化还原和免疫状态方面表现出比SS和SY更好的能力。
