Miguel Michelle A, Kim Seon-Ho, Lee Sang-Suk, Cho Yong-Il
Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Korea.
Animals (Basel). 2021 Oct 11;11(10):2937. doi: 10.3390/ani11102937.
The impact of soil with an intact microbial community and oxygen availability on moisture content, soil pH, and bacterial communities during decomposition of poultry carcasses was investigated. Poultry carcasses were decomposed in soil with or without a microbial community, under aerobic or anaerobic conditions. The samples collected in each microcosm burial set-up were analyzed by targeted 16S rRNA amplicon sequencing and Amplicon sequence variants (ASV) method. Our results showed that moisture was high in the burial set-ups under anaerobic conditions and pH was high in the burial set-ups under aerobic conditions. Meanwhile, the Chao1 and Shannon index significantly differed between the different burial set-ups and across different time points. In addition, bacterial taxa composition during the early period of decomposition differed from that of the late period. A total of 23 phyla, 901 genera, and 1992 species were identified. Firmicutes was the most dominant phyla in all burial set-ups throughout the decomposition. At day 60, was dominant in the burial set-ups under aerobic conditions, while dominated in the burial set-ups under anaerobic conditions. This study demonstrated that the soil microbial community and availability of oxygen significantly affected the changes in moisture content, pH, and bacterial composition during the decomposition process.
研究了具有完整微生物群落和氧气供应的土壤对家禽尸体分解过程中土壤湿度、pH值和细菌群落的影响。家禽尸体在有或没有微生物群落的土壤中，在有氧或厌氧条件下进行分解。对每个微观埋葬设置中收集的样本采用靶向16S rRNA扩增子测序和扩增子序列变体（ASV）方法进行分析。我们的结果表明，厌氧条件下的埋葬设置中湿度较高，有氧条件下的埋葬设置中pH值较高。同时，不同埋葬设置之间以及不同时间点的Chao1和香农指数存在显著差异。此外，分解早期的细菌分类群组成与后期不同。共鉴定出23个门、901个属和1992个物种。在整个分解过程中，厚壁菌门是所有埋葬设置中最主要的门。在第60天，有氧条件下的埋葬设置中 占主导地位，而厌氧条件下的埋葬设置中 占主导地位。本研究表明，土壤微生物群落和氧气供应显著影响了分解过程中土壤湿度、pH值和细菌组成的变化。
