Nieto-Velázquez Nayeli G, Gomez-Valdez Alejandra A, González-Ávila Marisela, Sánchez-Navarrete Jaime, Toscano-Garibay Julia D, Ruiz-Pérez Nancy J
Dirección de Investigación y Enseñanza, Hospital Juárez de México, Ciudad de México 07760, Mexico.
Facultad de Estudios Superiores Cuatitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli 54740, Mexico.
Antibiotics (Basel). 2021 Oct 7;10(10):1218. doi: 10.3390/antibiotics10101218.
Essential oils of and have shown biological functions as antiseptics, anti-inflammatories, antioxidants, antifungal and antimutagenic, so the evaluation of their antibacterial capacity, by themselves or in combination with standard antibiotics, presents an alternative for infection treatment. Flow cytometry opens the door for the design of faster and more accurate measurement of antibacterial activity. We use a SYTO9/PI staining system on ATCC 25922 to determine antibacterial activity by counting live and dead cells through flow cytometry. We found that dual staining showed highly variable results due to wavelength overlapping and instead we used fluorochrome individual staining that highly correlated with viable counts. Chloramphenicol and cefotaxime treatments did not present a dose-response behavior, rendered diffuse readings and/or gave filament formation on fluorescence microscopy. Amikacin was a better comparison standard because it presented a dose-response behavior. Essential oils had low antibacterial activity as compared to amikacin, with a maximum of 10% and 20% for and , respectively. Combinations of essential oils with antibiotic resulted in an unforeseen strong inhibition of amikacin activity. Although a low antibacterial activity was found, a series of standardization steps are proposed for antibacterial activity measurement by flow cytometry.
[植物名称1]和[植物名称2]的精油已显示出作为防腐剂、抗炎剂、抗氧化剂、抗真菌剂和抗诱变剂的生物学功能，因此评估它们自身或与标准抗生素联合使用时的抗菌能力，为感染治疗提供了一种替代方法。流式细胞术为设计更快、更准确的抗菌活性测量方法打开了大门。我们在[细菌名称]ATCC 25922上使用SYTO9/PI染色系统，通过流式细胞术对活细胞和死细胞进行计数来确定抗菌活性。我们发现，由于波长重叠，双重染色显示出高度可变的结果，因此我们改用与活菌计数高度相关的荧光染料单独染色。氯霉素和头孢噻肟处理未呈现剂量反应行为，在荧光显微镜下读数模糊和/或出现丝状形成。阿米卡星是一个更好的比较标准，因为它呈现出剂量反应行为。与阿米卡星相比，精油的抗菌活性较低，[植物名称1]和[植物名称2]的精油分别最高为10%和20%。精油与抗生素的组合导致对阿米卡星活性产生了意想不到的强烈抑制。尽管发现抗菌活性较低，但仍提出了一系列用于通过流式细胞术测量抗菌活性的标准化步骤。
