Ma Boheng, Wang Xuechun, Lei Changwei, Tang Yizhi, He Juan, Gao Yufeng, Zhang Yu, Wang Hongning
College of Life Sciences, Sichuan University, Chengdu 610065, China.
Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Sichuan University, Chengdu 610065, China.
Antibiotics (Basel). 2021 Oct 18;10(10):1268. doi: 10.3390/antibiotics10101268.
The widespread use of antibiotics in large-scale livestock production has led to serious antibiotic resistance. is an important pathogenic bacterium on large-scale farms. Chromosomally localized mobilizable genetic elements (genomic islands) and mobile genetic elements (Tn-like transposons) play an important role in the acquisition and transmission of resistance genes by . To study the prevalence and resistance characteristics of antibiotic-resistant genomic islands in of animal origin in China, we performed whole genome sequencing of isolated from large-scale pig and chicken farms. Three new variants of PmGRI1 (HN31, YN8, and YN9), and a hybrid structure (HN2p) formed by the multidrug-resistant Tn-like-HN2p transposon and a genomic island PmGRI1-HN2p, were identified from . All variants underwent homologous recombination mediated by insertion sequence IS. A genomic rearrangement in the chromosome between the Tn-like-HN2p transposon and PmGRI1-HN2p occurred in HN2p. The heterozygous structure contained various antimicrobial resistance genes, including three copies of fluoroquinolone resistance gene and 16S rRNA methylase gene , which are rarely found in . Our results highlight the structural genetic diversity of genomic islands by characterizing the novel variants of PmGRI1 and enrich the research base of multidrug resistance genomic islands.
抗生素在大规模牲畜养殖中的广泛使用已导致严重的抗生素耐药性。[细菌名称]是大型养殖场中的一种重要病原菌。染色体定位的可移动遗传元件（基因组岛）和移动遗传元件（Tn样转座子）在[细菌名称]获取和传播耐药基因方面发挥着重要作用。为了研究中国动物源[细菌名称]中抗生素耐药基因组岛的流行情况和耐药特征，我们对从大型猪场和鸡场分离出的[细菌名称]进行了全基因组测序。从[细菌名称]中鉴定出了PmGRI1的三个新变体（HN31、YN8和YN9），以及由多药耐药Tn样-HN2p转座子和基因组岛PmGRI1-HN2p形成的杂交结构（HN2p）。所有变体都经历了由插入序列IS介导的同源重组。在HN2p中，Tn样-HN2p转座子和PmGRI1-HN2p之间的染色体发生了基因组重排。该杂合结构包含各种抗菌耐药基因，包括三个拷贝的氟喹诺酮耐药基因[基因名称]和16S rRNA甲基化酶基因[基因名称]，这些基因在[细菌名称]中很少见。我们的结果通过表征PmGRI1的新变体突出了基因组岛的结构遗传多样性，并丰富了多药耐药基因组岛的研究基础。
