The School of Basic Medical Science and Public Center of Experimental Technology, Southwest Medical University, Luzhou, Sichuan, China.
Department of Clinical Laboratory, The Hejiang People's hospital, Luzhou, Sichuan, China.
Front Cell Infect Microbiol. 2023 Aug 11;13:1229194. doi: 10.3389/fcimb.2023.1229194. eCollection 2023.
is the one of most important pathogens of catheter-associated urinary tract infections. The emergence of multidrug-resistant (MDR) severely limits antibiotic treatments, which poses a public health risk. This study aims to investigate the resistance characteristics and virulence potential for a collection of clinical isolates.
Antibiotic susceptibility testing revealed fourteen MDR strains, which showed high resistance to most β-lactams and trimethoprim/sulfamethoxazole, and a lesser extent to quinolones. All the MDR strains were sensitive to carbapenems (except imipenem), ceftazidime, and amikacin, and most of them were also sensitive to aminoglycosides. The obtained MDR isolates were sequenced using an Illumina HiSeq. The core genome-based phylogenetic tree reveals the high genetic diversity of these MDR isolates and highlights the possibility of clonal spread of them across China. Mobile genetic elements SXT/R391 ICEs were commonly (10/14) detected in these MDR strains, whereas the presence of resistance island GRI1 and plasmid was sporadic. All ICEs except for ICEChn31006 carried abundant antimicrobial resistance genes (ARGs) in the HS4 region, including the extended-spectrum β-lactamase (ESBL) gene . ICEChn31006 contained the sole ARG and was nearly identical to the global epidemic ICEPmiJpn1. The findings highlight the important roles of ICEs in mediating the spread of ARGs in strains. Additionally, these MDR strains have great virulence potential as they exhibited significant virulence-related phenotypes including strong crystalline biofilm, hemolysis, urease production, and robust swarming motility, and harbored abundant virulence genes.
In conclusion, the prevalence of MDR with high virulence potential poses an urgent threat to public health. Intensive monitoring is needed to reduce the incidence of infections by MDR .
是引起导管相关性尿路感染的最重要病原体之一。多药耐药（MDR）的出现严重限制了抗生素治疗，这对公共健康构成了威胁。本研究旨在研究一组临床分离株的耐药特征和毒力潜力。
抗生素敏感性测试显示，有 14 株 MDR 菌株，它们对大多数β-内酰胺类和甲氧苄啶/磺胺甲恶唑表现出高度耐药，对喹诺酮类的耐药性较低。所有 MDR 菌株均对碳青霉烯类（除亚胺培南外）、头孢他啶和阿米卡星敏感，大多数菌株对氨基糖苷类也敏感。使用 Illumina HiSeq 对获得的 MDR 分离株进行测序。基于核心基因组的系统发育树显示，这些 MDR 分离株具有很高的遗传多样性，并突出了它们在中国境内克隆传播的可能性。SXT/R391 ICEs 等移动遗传元件在这些 MDR 菌株中经常（10/14）检测到，而抗性岛 GRI1 和质粒的存在则是零星的。除了 ICEChn31006 之外，所有 ICEs 在 HS4 区域都携带了大量的抗生素耐药基因（ARGs），包括超广谱β-内酰胺酶（ESBL）基因。ICEChn31006 仅含有一个 ARG，与全球流行的 ICEPmiJpn1 几乎相同。这些发现强调了 ICEs 在介导 ARGs 在 株中的传播方面的重要作用。此外，这些 MDR 菌株具有很强的毒力潜力，因为它们表现出显著的与毒力相关的表型，包括强烈的结晶生物膜、溶血、脲酶产生和强大的群集运动能力，并携带丰富的毒力基因。
总之，高毒力潜力的 MDR 的流行对公共健康构成了紧迫威胁。需要加强监测，以减少 MDR 感染的发生率。
