Jing Aihua, Liang Gaofeng, Yuan Yixin, Feng Wenpo
School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471003, China.
Medical College, Henan University of Science and Technology, Luoyang 471003, China.
Micromachines (Basel). 2019 Jan 24;10(2):84. doi: 10.3390/mi10020084.
The quantification of ascorbic acid (AA), dopamine (DA), and uric acid (UA) has been an important area of research, as these molecules' determination directly corresponds to the diagnosis and control of diseases of nerve and brain physiology. In our research, graphene oxide (GO) with nano pores deposited with gold nanoparticles were self-assembled to form three-dimensional (3D) Au/holey-graphene oxide (Au/HGO) composite structures. The as-prepared 3DAu/HGO composite structures were characterized for their structures by X-ray diffraction, Raman spectrum, scanning electron microscopy, and transmission electron microscopy coupled with cyclic voltammograms. Finally, the proposed 3DAu/HGO displayed high sensitivity, excellent electron transport properties, and selectivity for the simultaneous electrochemical determination of AA, DA and UA with linear response ranges of 1.0⁻500 μM, 0.01⁻50 μM and 0.05⁻50 μM respectively. This finding paves the way for graphene applications as a biosensor for detecting three analytes in human serum.
抗坏血酸（AA）、多巴胺（DA）和尿酸（UA）的定量分析一直是一个重要的研究领域，因为这些分子的测定直接关系到神经和大脑生理学疾病的诊断与控制。在我们的研究中，沉积有金纳米颗粒的纳米孔氧化石墨烯（GO）自组装形成三维（3D）金/多孔氧化石墨烯（Au/HGO）复合结构。通过X射线衍射、拉曼光谱、扫描电子显微镜、透射电子显微镜以及循环伏安图对所制备的3D Au/HGO复合结构的结构进行了表征。最后，所提出的3D Au/HGO对AA、DA和UA的同时电化学测定显示出高灵敏度、优异的电子传输性能和选择性，其线性响应范围分别为1.0⁻500 μM、0.01⁻50 μM和0.05⁻50 μM。这一发现为石墨烯作为检测人血清中三种分析物的生物传感器的应用铺平了道路。
