Jing Aihua, Xu Qiong, Feng Wenpo, Liang Gaofeng
School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, China.
Medical College, Henan University of Science and Technology, Luoyang 471023, China.
Micromachines (Basel). 2020 Jul 3;11(7):660. doi: 10.3390/mi11070660.
Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. The quantitative detection of a low concentration of CEA has important medical significance. In this study, three-dimensional porous graphene-oxide-supported platinum metal nanoparticles (3DPt/HGO) composites were prepared by a wet chemical method and modified on an electrode with enhanced conductivity, a large surface area, and good adsorption of immobilizing antibodies (Ab). Horseradish peroxidase (HRP)-functionalized Au nanoparticles were fabricated to label the secondary antibodies (Ab). The proposed immunosensor showed a good linear relationship in the range of 0.001-150 ng/mL for CEA and a detection limit of 0.0006 ng/mL. The immunosensor had high sensitivity, good stability and reproducibility, and has great application prospects for the clinical diagnosis of cancer.
癌胚抗原（CEA）是一种重要的广谱肿瘤标志物。低浓度CEA的定量检测具有重要的医学意义。在本研究中，通过湿化学方法制备了三维多孔氧化石墨烯负载铂金属纳米颗粒（3DPt/HGO）复合材料，并将其修饰在具有增强导电性、大表面积和良好抗体固定吸附性的电极上。制备了辣根过氧化物酶（HRP）功能化的金纳米颗粒来标记二抗（Ab）。所提出的免疫传感器在0.001 - 150 ng/mL范围内对CEA呈现良好的线性关系，检测限为0.0006 ng/mL。该免疫传感器具有高灵敏度、良好的稳定性和重现性，在癌症临床诊断方面具有广阔的应用前景。
