Sinno Martina, Ranesi Marta, Di Lelio Ilaria, Iacomino Giuseppina, Becchimanzi Andrea, Barra Eleonora, Molisso Donata, Pennacchio Francesco, Digilio Maria Cristina, Vitale Stefania, Turrà David, Harizanova Vili, Lorito Matteo, Woo Sheridan Lois
Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy.
BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples Federico II, 80055 Naples, Italy.
Pathogens. 2021 Sep 26;10(10):1242. doi: 10.3390/pathogens10101242.
Endophytic fungi (EF) can enhance both plant growth and defense barriers against pests and pathogens, contributing to the reduction of chemical pesticides and fertilizers use in agriculture. is an entomopathogenic fungus showing endophytism in several crops, often associated with a good capacity to limit the development of pests and disease agents. However, the diversity of the protective efficacy and plant response to different strains can be remarkable and needs to be carefully assessed for the successful and predictable use of these beneficial microorganisms. This study aims to select strains able to colonize tomato plants as endophytes as well as to control two important disease agents, and and the pest aphid, . Nine wild-type isolates and one commercial strain were screened for endophytism, then further characterized for plant-growth promotion plus inhibition of disease development and pest infestation. Four isolates proved to have a good control activity against the biotic stressors tested, but only Bb716 was also able to promote plant growth. This work provides a simple workflow for the selection of beneficial EF, paving the way towards more effective use of in Integrate Pest Management (IPM) of tomato.
内生真菌（EF）可以促进植物生长，并增强植物对害虫和病原体的防御屏障，有助于减少农业中化学农药和化肥的使用。绿僵菌是一种在几种作物中表现出内生性的昆虫病原真菌，通常具有良好的限制害虫和病原体发展的能力。然而，不同菌株的保护效果和植物反应的多样性可能非常显著，为了成功且可预测地使用这些有益微生物，需要仔细评估。本研究旨在筛选能够作为内生菌定殖在番茄植株上的菌株，同时控制两种重要的病原体——番茄早疫病菌和番茄灰霉病菌以及害虫蚜虫。对9个野生型分离株和1个商业菌株进行了内生性筛选，然后进一步对其促进植物生长以及抑制病害发展和害虫侵染的特性进行了表征。4个分离株对所测试的生物胁迫因子具有良好的控制活性，但只有Bb716还能够促进植物生长。这项工作为筛选有益内生真菌提供了一个简单的工作流程，为在番茄病虫害综合防治（IPM）中更有效地利用绿僵菌铺平了道路。
