Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.
NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Molecules. 2021 Oct 10;26(20):6102. doi: 10.3390/molecules26206102.
Chemical compositions, antioxidants, and anti-aging activities of Cortex Moutan (CM), from different collection periods and different producing areas, were measured and compared in order to obtain excellent CM extracts. The bioactivities of CM extracts were examined by an in vitro antioxidant method and a UVB irradiated human dermal fibroblast (HDF) model. Phytochemical properties were obtained from ultra-fast liquid chromatography quadrupole time-of-flight mass spectrometry (UFLC-Q-TOF-MS) prior to the multivariate statistical analysis. As for the results, the extracts of Heze CM (HZCM) and Luoyang CM (LYCM) collected in June had better in vitro antioxidant activities, significantly increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and reduced the content of malondialdehyde (MDA), compared to other CM extracts. HZCM and LYCM extracts could upregulate the relative expression of SOD and GSH-Px mRNA. The extract of HZCM collected in June could significantly repress the production of matrix metalloproteinase 1 (MMP-1) and improve the production of procollagen type I (PCOL)-I in UVB irradiated HDF. In total, 50 compounds, including 17 monoterpenoids, 19 flavonoids, 13 phenols, and 1 amino acid were identified or tentatively identified in the CM extracts. Gallic acid, -hydroxybenzoic acid, oxypaeoniflorin, paeoniflorin, 1,2,3,4,6--pentagalloyl glucose, and paeonol were predominant compounds in the CM extracts. Taken together, CM collected from April to September had better antioxidant and anti-aging effects for external usage.
为了获得优质的牡丹皮提取物，对来自不同采集期和不同产地的牡丹皮（CM）的化学成分、抗氧化剂和抗衰老活性进行了测量和比较。通过体外抗氧化方法和 UVB 辐射的人真皮成纤维细胞（HDF）模型来检测 CM 提取物的生物活性。在进行多变量统计分析之前，通过超快速液相色谱四极杆飞行时间质谱（UFLC-Q-TOF-MS）获得植物化学成分。结果表明，6 月采集的菏泽牡丹皮（HZCM）和洛阳牡丹皮（LYCM）提取物具有更好的体外抗氧化活性，与其他 CM 提取物相比，显著提高了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性，并降低了丙二醛（MDA）的含量。HZCM 和 LYCM 提取物可上调 SOD 和 GSH-Px mRNA 的相对表达。6 月采集的 HZCM 提取物可显著抑制基质金属蛋白酶 1（MMP-1）的产生，并改善 UVB 辐射的 HDF 中 I 型前胶原（PCOL-I）的产生。总共鉴定或初步鉴定了 50 种化合物，包括 17 种单萜、19 种黄酮类化合物、13 种酚类化合物和 1 种氨基酸。在 CM 提取物中，鉴定出的主要化合物有没食子酸、-羟基苯甲酸、氧化芍药苷、芍药苷、1,2,3,4,6--五没食子酰葡萄糖和丹皮酚。总之，4 月至 9 月采集的牡丹皮具有更好的抗氧化和抗衰老作用，可用于外部使用。
