Barbaresco Federica, Racca Luisa, Spigarelli Luca, Cocuzza Matteo, Marasso Simone Luigi, Pirri Candido Fabrizio, Canavese Giancarlo
Chilab-Materials and Microsystems Laboratory, DISAT, Politecnico di Torino, Chivasso, 10034 Turin, Italy.
Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy.
Nanomaterials (Basel). 2021 Oct 6;11(10):2630. doi: 10.3390/nano11102630.
This work illustrates focalization performances of a silicon-based bulk acoustic wave device applied for the separation of specimens owing to micrometric dimensions. Samples are separated in the microfluidic channel by the presence of an acoustic field, which focalizes particles or cells according to their mechanical properties compared to the surrounded medium ones. Design and fabrication processes are reported, followed by focalization performance tests conducted either with synthetic particles or cells. High focalization performances occurred at different microparticle concentrations. In addition, preliminary tests carried out with HL-60 cells highlighted an optimal separation performance at a high flow rate and when cells are mixed with micro and nanoparticles without affecting device focalization capabilities. These encouraging results showed how this bulk acoustic wave device could be exploited to develop a diagnostic tool for early diagnosis or some specific target therapies by separating different kinds of cells or biomarkers possessing different mechanical properties such as shapes, sizes and densities.
这项工作展示了一种基于硅的体声波器件的聚焦性能，该器件由于其微米级尺寸而应用于样本分离。在微流体通道中，通过声场的存在对样本进行分离，声场根据颗粒或细胞与周围介质相比的机械特性使它们聚焦。报告了设计和制造工艺，随后进行了使用合成颗粒或细胞的聚焦性能测试。在不同的微粒浓度下都出现了高聚焦性能。此外，用HL-60细胞进行的初步测试突出表明，在高流速下以及细胞与微米和纳米颗粒混合时，能实现最佳分离性能，且不会影响器件的聚焦能力。这些令人鼓舞的结果表明，这种体声波器件可用于开发一种诊断工具，通过分离具有不同机械特性（如形状、大小和密度）的不同种类细胞或生物标志物，实现早期诊断或某些特定的靶向治疗。
