Wang Min, Xie Xueheng, Du Yuyang, Ma Guoxu, Xu Xudong, Sun Guibo, Sun Xiaobo
Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine (Natural Medicine) and Translational Medicine, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, 100193 Beijing, China.
Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, 150076 Harbin, China.
Evid Based Complement Alternat Med. 2020 May 18;2020:2162915. doi: 10.1155/2020/2162915. eCollection 2020.
Leukopenia, a common complication of tumor chemoradiotherapy, contributes serious damage to the hematopoietic, gastrointestinal, and immune systems of the body and can cause delay, discontinuation, or even failure to tumor treatment, thereby greatly threatening human health. The present study aims to investigate the protective effects of biscoclaurine alkaloids (BA) on leukopenia.
This study was conducted on 60 Kunming mice, which were randomly divided into six groups containing 10 animals each. A hematology analyzer was used to count white blood cells (WBC) in the peripheral blood cell. Mice serum was collected, and the granulocyte-macrophage colony-stimulating factor, vascular cell adhesion molecule 1 (VCAM-1), and interferon- (IFN-) were detected by enzyme-linked immunosorbent assays. Pathological changes were detected through hematoxylin and eosin staining in the liver and spleen of mice. The spleen and liver ultrastructures were observed via electron microscopy.
Results showed that BA ameliorated WBC, PLT reduction in the peripheral blood and significantly increased the levels of IFN- and VCAM-1 in mice serum. BA reduced ionizing radiation-induced injuries to spleen, mitigated the reduction of superoxide dismutase (SOD), and significantly decreased the malonaldehyde (MDA) and xanthine oxidase (XOD) levels in the liver.
BA enhanced the immune and hematopoietic functions and ameliorated the oxidative stress induced by Co- radiation, revealing its therapeutic potential both as a radioprotector and as a radiation mitigator for leukopenia induced by Co- radiation.
白细胞减少是肿瘤放化疗常见的并发症，对机体的造血、胃肠及免疫系统造成严重损害，可导致肿瘤治疗延迟、中断甚至治疗失败，从而极大地威胁人类健康。本研究旨在探讨双苄基异喹啉生物碱（BA）对白细胞减少的保护作用。
本研究选用60只昆明小鼠，随机分为6组，每组10只。采用血液学分析仪计数外周血细胞中的白细胞（WBC）。采集小鼠血清，采用酶联免疫吸附测定法检测粒细胞巨噬细胞集落刺激因子、血管细胞黏附分子1（VCAM-1）和干扰素-（IFN-）。通过苏木精和伊红染色检测小鼠肝脏和脾脏的病理变化。通过电子显微镜观察脾脏和肝脏的超微结构。
结果显示，BA改善了外周血白细胞、血小板的减少，并显著提高了小鼠血清中IFN-和VCAM-1的水平。BA减轻了电离辐射对脾脏的损伤，减轻了超氧化物歧化酶（SOD）的降低，并显著降低了肝脏中丙二醛（MDA）和黄嘌呤氧化酶（XOD）的水平。
BA增强了免疫和造血功能，改善了钴辐射诱导的氧化应激，揭示了其作为辐射防护剂和钴辐射诱导的白细胞减少的辐射减轻剂的治疗潜力。
