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转录组和代谢组分析为根状茎形成过程提供了新的见解。

Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of Roots.

机构信息

College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.

Institute for Processing and Storage of Agricultural Products, Chengdu Academy of Agricultural and Forest Sciences, Chengdu 611130, China.

出版信息

Int J Mol Sci. 2022 Jun 7;23(12):6390. doi: 10.3390/ijms23126390.

DOI:10.3390/ijms23126390
PMID:35742832
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9224348/
Abstract

Chayote produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. 'Tuershao', a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization.

摘要

瓠瓜生长 1 年后可产生具有高淀粉含量的可食用块根，但瓠瓜块根形成的机制尚不清楚。“特少”是一种缺乏可食用果实但比传统瓠瓜品种具有更高块根产量的瓠瓜品种，用于通过对三个块根生长阶段的代谢组和转录组谱进行综合分析来研究块根形成。在块根膨大过程中，与淀粉生物合成和半乳糖代谢相关的基因和代谢物显著上调，而编码糖最终将被输出的转运蛋白（SWEET）和糖转运蛋白（SUT）在块根形成过程中高度表达。生长素前体（吲哚-3-乙酰胺）和乙烯前体 1-氨基环丙烷-1-羧酸被上调，表明这两种激素在块根发育和成熟中起关键作用。我们的数据揭示了瓠瓜与土豆类似的块根形成信号通路，包括 BEL1/KNOX 和 SP6A/14-3-3/FDL 复合物。BEL1/KNOX 复合物的下调和 14-3-3 蛋白的上调表明这两个复合物在块根形成中可能具有不同的功能。最后，基因表达和显微镜分析表明在块根形成的初始阶段存在活跃的细胞分裂。总之，转录组和代谢组分析的整合揭示了瓠瓜块根形成的整体分子网络，这可能有助于其利用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b038/9224348/9c13889e9b9b/ijms-23-06390-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b038/9224348/bec153d5a33b/ijms-23-06390-g001.jpg
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