Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, 450001 Zhengzhou, China.
Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, 450001 Zhengzhou, China; State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Int J Biol Macromol. 2021 Dec 15;193(Pt A):823-837. doi: 10.1016/j.ijbiomac.2021.10.079. Epub 2021 Oct 20.
Cystathionine β-synthase (CBS) domains containing proteins (CDCPs) form a large family and play roles in development via regulation of the thioredoxin system as well as abiotic and biotic stress responses of plant. However, the comprehensive study of CBS genes remained elusive in cotton. Here, we identified 237 CBS genes in 11 plant species and the phylogenetic analysis categorized CBS genes into four groups. Whole-genome or segmental with dispersed duplication events contributed to GhCBS gene family expansion. Moreover, orthologous/paralogous genes among three cotton species (G. hirsutum, G. arboreum, and G. raimondii) were detected from the syntenic map among eight plant species. Strong purifying selection for dicotyledonous and monocotyledonous CBS genes, and cis-elements related to plant growth and development, abiotic and hormonal response were observed. Transcriptomic data and qRT-PCR validation of 12 GhCBS genes indicated their critical role in ovule development as most of the genes showed high enrichment. Further, some of GhCBS (GhCBS5, GhCBS16, GhCBS17, GhCBS24, GhCBS25, GhCBS26, and GhCBS52) genes were regulated under various abiotic and hormonal treatments for different time points and involve in ovule and fiber development which provided key genes for future cotton breeding programs. In addition, transgenic tobacco plants overexpressing GhCBS4 transiently exhibited higher water and chlorophyll content indicating improved tolerance toward drought stress. Overall, this study provides the characterization of GhCBS genes for plant growth, abiotic and hormonal stresses, thereby, intimating their significance in cotton molecular breeding for resistant cultivars.
半胱氨酸β-合酶（CBS）结构域包含蛋白（CDCPs）形成一个大家族，通过调节硫氧还蛋白系统以及植物的非生物和生物胁迫反应，在发育中发挥作用。然而，棉纤维中 CBS 基因的综合研究仍然难以捉摸。在这里，我们在 11 种植物物种中鉴定出 237 个 CBS 基因，系统发育分析将 CBS 基因分为四个组。全基因组或片段的分散重复事件有助于 GhCBS 基因家族的扩张。此外，在 8 种植物的共线性图谱中检测到三种棉花物种（G. hirsutum、G. arboreum 和 G. raimondii）之间的直系/旁系同源基因。观察到双子叶植物和单子叶植物 CBS 基因的强烈纯化选择，以及与植物生长发育、非生物和激素反应相关的顺式元件。12 个 GhCBS 基因的转录组数据和 qRT-PCR 验证表明，它们在胚珠发育中起着关键作用，因为大多数基因表现出高度富集。此外，一些 GhCBS（GhCBS5、GhCBS16、GhCBS17、GhCBS24、GhCBS25、GhCBS26 和 GhCBS52）基因在不同的非生物和激素处理下在不同的时间点被调控，参与胚珠和纤维发育，为未来的棉花育种计划提供了关键基因。此外，过表达 GhCBS4 的转基因烟草植物在瞬时表现出更高的水分和叶绿素含量，表明对干旱胁迫的耐受性提高。总的来说，这项研究为植物生长、非生物和激素胁迫提供了 GhCBS 基因的特征，从而暗示了它们在棉花分子育种中对抗性品种的重要性。
