Li Jisheng, Zhang Bowen, Gan Min, Li Yunxing, He Lijuan, Yue Wen, Qiao Haixuan, Pei Xuetao, Li Yanhua
Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion Medicine, Beijing 100850, China.
Beijing Institute of Radiation Medicine, Beijing 100850, China; South China Research Center for Stem Cell & Regenerative Medicine, SCIB, Guangzhou 510005, China.
Stem Cell Res. 2021 Dec;57:102581. doi: 10.1016/j.scr.2021.102581. Epub 2021 Oct 19.
Serine hydroxymethyltransferase 2 (SHMT2), a catalytic enzyme playing an important role in aerobic cellular respiration and mitochondrial metabolism, might be pivotal in self-renewal and differentiation of human pluripotent stem cells. Herein, we used the CRISPR/Cas9 editing system to construct a homozygous SHMT2 knockout (SHMT2-KO) human embryonic stem cell (hESC) line, exhibiting a normal karyotype, colony morphology, and high expression levels of pluripotent proteins. Furthermore, SHMT2 knockout did not impact the self-renewal ability or differentiation potential into three germ layers of hESCs. Accordingly, this cell line provides a valuable model for further assessing SHMT2 functions in human embryonic development.
丝氨酸羟甲基转移酶2（SHMT2）是一种在有氧细胞呼吸和线粒体代谢中起重要作用的催化酶，可能在人类多能干细胞的自我更新和分化中起关键作用。在此，我们使用CRISPR/Cas9编辑系统构建了一个纯合的SHMT2基因敲除（SHMT2-KO）人类胚胎干细胞（hESC）系，该细胞系具有正常的核型、集落形态以及多能蛋白的高表达水平。此外，SHMT2基因敲除并不影响hESC的自我更新能力或向三个胚层的分化潜能。因此，该细胞系为进一步评估SHMT2在人类胚胎发育中的功能提供了一个有价值的模型。
