Wang Ruijie, Qin Yuan, Liu Xin, Li Yangzi, Lin Zhenfeng, Nie Rong, Shi Yifei, Huang Huayu
Shaanxi Key Laboratory of Earth Surface System and Environmental Carrying Capacity, College of Urban and Environmental Science, Northwest University, Xi'an 710127, China.
Key Laboratory of Watershed Geographic Sciences, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China.
ACS Omega. 2021 Oct 6;6(41):27405-27411. doi: 10.1021/acsomega.1c04326. eCollection 2021 Oct 19.
A selective electrochemical biosensor was developed for detecting carbendazim (CBZ) based on well-dispersed colloidal boron nitride (BN) nanocrystals and gold nanoparticles (Au NPs). BN was synthesized by "solvent cutting" to modify a glassy carbon electrode (GCE), and Au NPs were then electrodeposited. A single-stranded oligonucleotide with methylene blue (MB) was modified to the electrode surface through gold-sulfur bonds. A double-stranded DNA was formed in the presence of an aptamer. The aptamer chain can specifically bind to the target CBZ. When the aptamer binds to CBZ, the electroactive substance MB labeled at one end of the complementary chain can effectively contact the electrode surface. Detection of CBZ is realized by simultaneously monitoring the MB signal enhancement. The CBZ concentration was determined in a wide linearity range from 0.1 ng mL to 100 μg mL, with a low detection limit of 0.019 ng mL. This biosensor exhibited excellent selectivity and acceptable repeatability and was applied in cucumber, kiwifruit, and water samples with good recoveries, demonstrating that the strategy has remarkable potential and offers a good platform for CBZ detection.
基于分散良好的胶体氮化硼（BN）纳米晶体和金纳米颗粒（Au NPs），开发了一种用于检测多菌灵（CBZ）的选择性电化学生物传感器。通过“溶剂切割”合成BN以修饰玻碳电极（GCE），然后电沉积Au NPs。通过金硫键将带有亚甲基蓝（MB）的单链寡核苷酸修饰到电极表面。在适体存在下形成双链DNA。适体链可以特异性结合目标CBZ。当适体与CBZ结合时，标记在互补链一端的电活性物质MB可以有效地接触电极表面。通过同时监测MB信号增强来实现对CBZ的检测。CBZ浓度在0.1 ng/mL至100 μg/mL的宽线性范围内测定，检测限低至0.019 ng/mL。该生物传感器具有优异的选择性和可接受的重复性，并应用于黄瓜、猕猴桃和水样中，回收率良好，表明该策略具有显著潜力，为CBZ检测提供了一个良好的平台。
