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通过膜片变形镜的连续振荡实现视频速率的远程重新聚焦。

Video-rate remote refocusing through continuous oscillation of a membrane deformable mirror.

作者信息

Wright Terry, Sparks Hugh, Paterson Carl, Dunsby Chris

机构信息

Photonics Group, Department of Physics, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom.

Centre for Pathology, Department of Medicine, Imperial College London, Du Cane Road, London W12 0NN, United Kingdom.

出版信息

JPhys Photonics. 2021 Oct;3(4):045004. doi: 10.1088/2515-7647/ac29a2. Epub 2021 Oct 20.

DOI:10.1088/2515-7647/ac29a2
PMID:34693207
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8523955/
Abstract

This paper presents the use of a deformable mirror (DM) configured to rapidly refocus a microscope employing a high numerical aperture (NA) objective lens. An Alpao DM97-15 membrane DM was used to refocus a 40×/0.80 NA water-immersion objective through a defocus range of -50-50 m at 26.3 sweeps s. We achieved imaging with a mean Strehl metric of >0.6 over a field of view in the sample of 200 × 200 m over a defocus range of 77 m. We describe an optimisation procedure where the mirror is swept continuously in order to avoid known problems of hysteresis associated with the membrane DM employed. This work demonstrates that a DM-based refocusing system could in the future be used in light-sheet fluorescence microscopes to achieve video-rate volumetric imaging.

摘要

本文介绍了一种可变形镜（DM）的应用，该可变形镜被配置为使用高数值孔径（NA）物镜快速重新聚焦显微镜。使用Alpao DM97 - 15膜片式可变形镜，通过在26.3次扫描/秒的速度下在-50至50微米的离焦范围内对40×/0.80 NA水浸物镜进行重新聚焦。在77微米的离焦范围内，我们在200×200微米的样本视场中实现了平均斯特列尔比大于0.6的成像。我们描述了一种优化程序，其中镜子连续扫描以避免与所采用的膜片式可变形镜相关的已知滞后问题。这项工作表明，基于可变形镜的重新聚焦系统未来可用于光片荧光显微镜，以实现视频速率的体积成像。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/68490b3cec98/jpphotonac29a2f8_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/497925184c6d/jpphotonac29a2f1_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/74bc33e77c58/jpphotonac29a2f2_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/745926cf8b06/jpphotonac29a2f3_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/503b372fe558/jpphotonac29a2f4_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/a458dc1fe55b/jpphotonac29a2f5_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/21bb15b31bb1/jpphotonac29a2f6_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/427272eaa5a0/jpphotonac29a2f7_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/68490b3cec98/jpphotonac29a2f8_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/497925184c6d/jpphotonac29a2f1_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/74bc33e77c58/jpphotonac29a2f2_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/745926cf8b06/jpphotonac29a2f3_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/503b372fe558/jpphotonac29a2f4_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/a458dc1fe55b/jpphotonac29a2f5_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/21bb15b31bb1/jpphotonac29a2f6_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/427272eaa5a0/jpphotonac29a2f7_lr.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/339b/8523955/68490b3cec98/jpphotonac29a2f8_lr.jpg

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Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens.
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J Biomed Opt. 2024 Mar;29(3):036502. doi: 10.1117/1.JBO.29.3.036502. Epub 2024 Mar 21.
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Alignment and characterization of remote-refocusing systems.远程重新聚焦系统的校准与特性描述
Appl Opt. 2023 Oct 1;62(28):7431-7440. doi: 10.1364/AO.500281.
用具有像差校正功能的自适应透镜对厚生物组织进行衍射极限轴向扫描。
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