College of Chemistry and Environmental Science, Inner Mongolia Key Laboratory of Environmental Chemistry, Inner Mongolia Normal University, Hohhot, China.
College of Pharmacy, Inner Mongolia Medical University, Hohhot, China.
IET Nanobiotechnol. 2021 Jul;15(5):512-518. doi: 10.1049/nbt2.12049. Epub 2021 Mar 22.
Silver nanomaterials have become important research topics in recent years. As a new type of fluorescent material, silver nanomaterials have been applied to fluorescent sensors, bioimaging and materials targeting cancer cells. Here, an approach to the oligonucleotide-templated controllable formation of fluorescent Ag nanomaterials is reported. In this experiment, silver nanoparticles (NPs) were synthesised from oligonucleotides chains, sodium borohydride (NaBH ) and silver nitrate (AgNO ) by changing the molar ratio of DNA to sodium borohydride (NaBH ) and silver nitrate (AgNO ). Fluorescent assay and transmission electron microscopy were used to characterise the silver NPs. The optimal selection of DNA chains with different lengths as templates for the synthesis of silver NPs was found. This work successfully develops the capping oligonucleotides scaffolds of silver nanoclusters.
近年来，纳米银材料已经成为研究的热点。作为一种新型的荧光材料，纳米银材料已被应用于荧光传感器、生物成像和针对癌细胞的靶向材料。本研究报告了一种基于寡核苷酸模板控制合成荧光 Ag 纳米材料的方法。在该实验中，通过改变 DNA 与硼氢化钠（NaBH4）和硝酸银（AgNO3）的摩尔比，从寡核苷酸链、硼氢化钠（NaBH4）和硝酸银（AgNO3）合成了银纳米粒子（NPs）。利用荧光测定法和透射电子显微镜对银纳米粒子进行了表征。实验发现，通过选择不同长度的 DNA 链作为模板来合成银 NPs，可以得到最佳的选择。这项工作成功地开发了银纳米团簇的盖帽寡核苷酸支架。
