State Key Laboratory for Agro-Biotechnology, Ministry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Viruses. 2021 Sep 24;13(10):1915. doi: 10.3390/v13101915.
Cercospora leaf spot (CLS) caused by is a devastating foliar disease of sugar beet (), resulting in high yield losses worldwide. Mycoviruses are widespread fungi viruses and can be used as a potential biocontrol agent for fugal disease management. To determine the presence of mycoviruses in , high-throughput sequencing analysis was used to determine the diversity of mycoviruses in 139 isolates collected from major sugar beet production areas in China. The high-throughput sequencing reads were assembled and searched against the NCBI database using BLASTn and BLASTx. The results showed that the obtained 93 contigs were derived from eight novel mycoviruses, which were grouped into 3 distinct lineages, belonging to the families and as well as some unclassified (-)ssRNA viruses in the order and . To the best of our knowledge, this is the first identification of highly diverse mycoviruses in The novel mycoviruses explored in this study will provide new viral materials to biocontrol Cercospora diseases. Future studies of these mycoviruses will aim to assess the roles of each mycovirus in biological function of in the future.
尾孢叶斑病（CLS）由 引起，是甜菜（）的一种毁灭性叶部病害，导致全球产量损失惨重。真菌病毒广泛存在，可作为真菌病害管理的潜在生物防治剂。为了确定 是否存在真菌病毒，我们使用高通量测序分析来确定从中国主要甜菜生产区采集的 139 个 分离物中真菌病毒的多样性。将高通量测序reads 组装并使用 BLASTn 和 BLASTx 与 NCBI 数据库进行搜索。结果表明，获得的 93 个重叠群来自 8 种新型真菌病毒，它们分为 3 个不同的谱系，属于家族 和 以及一些未分类的（-）ssRNA 病毒，在目 和 中。据我们所知，这是首次在 中鉴定出高度多样化的真菌病毒。本研究中探索的新型真菌病毒将为生物防治尾孢菌病提供新的病毒材料。未来将对这些真菌病毒进行研究，以评估每个真菌病毒在 生物学功能中的作用。
