Kim Geon Min, Sohn Hee Ju, Choi Won Seok, Sohn Uy Dong
Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea.
Department of Surgery, Chung-Ang University Hospital, Chung-Ang University, Seoul 06973, Korea.
Korean J Physiol Pharmacol. 2021 Nov 1;25(6):507-515. doi: 10.4196/kjpp.2021.25.6.507.
Postoperative ileus (POI), a symptom that occurs after abdominal surgery, reduces gastrointestinal motility. Although its mechanism is unclear, POI symptoms are known to be caused by inflammation 6 to 72 h after surgery. As proton pump inhibitors exhibit protective effect against acute inflammation, the purpose of this study was to determine the effect of ilaprazole on a POI rat model. POI was induced in rats by abdominal surgery. Rats were divided into six groups: control: normal rat + 0.5% CMC-Na, vehicle: POI rat + 0.5% CMC-Na, mosapride: POI rat + mosapride 2 mg/kg, ilaprazole 1 mg/kg: POI rat + ilaprazole 1 mg/kg, ilaprazole 3 mg/kg: POI rat + ilaprazole 3 mg/kg, and ilaprazole 10 mg/kg: POI rat + ilaprazole 10 mg/kg. Gastrointestinal motility was confirmed by measuring gastric emptying (GE) and gastrointestinal transit (GIT). In the small intestine, inflammation was confirmed by measuring TNF-α and IL-1β; oxidative stress was confirmed by SOD, GSH, and MDA levels; and histological changes were observed by H&E staining. Based on the findings, GE and GIT were decreased in the vehicle group and improved in the ilaprazole 10 mg/kg group. In the ilaprazole 10 mg/kg group, TNF-α and IL-1β levels were decreased, SOD and GSH levels were increased, and MDA levels were decreased. Histological damage was also reduced in the ilaprazole-treated groups. These findings suggest that ilaprazole prevents the decrease in gastrointestinal motility, a major symptom of postoperative ileus, and reduces inflammation and oxidative stress.
术后肠梗阻（POI）是腹部手术后出现的一种症状，会降低胃肠蠕动。尽管其机制尚不清楚，但已知POI症状是由术后6至72小时的炎症引起的。由于质子泵抑制剂对急性炎症具有保护作用，本研究的目的是确定伊索拉唑对POI大鼠模型的影响。通过腹部手术在大鼠中诱导POI。大鼠分为六组：对照组：正常大鼠 + 0.5% CMC-Na，赋形剂组：POI大鼠 + 0.5% CMC-Na，莫沙必利组：POI大鼠 + 莫沙必利2 mg/kg，伊索拉唑1 mg/kg组：POI大鼠 + 伊索拉唑1 mg/kg，伊索拉唑3 mg/kg组：POI大鼠 + 伊索拉唑3 mg/kg，伊索拉唑10 mg/kg组：POI大鼠 + 伊索拉唑10 mg/kg。通过测量胃排空（GE）和胃肠传输（GIT）来确认胃肠蠕动。在小肠中，通过测量TNF-α和IL-1β来确认炎症；通过超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和丙二醛（MDA）水平来确认氧化应激；通过苏木精-伊红（H&E）染色观察组织学变化。基于这些发现，赋形剂组的GE和GIT降低，而伊索拉唑10 mg/kg组有所改善。在伊索拉唑10 mg/kg组中，TNF-α和IL-1β水平降低，SOD和GSH水平升高，MDA水平降低。伊索拉唑治疗组的组织学损伤也有所减轻。这些发现表明，伊索拉唑可预防术后肠梗阻的主要症状——胃肠蠕动的降低，并减轻炎症和氧化应激。
