Lawrence Lincy, Menon Seema, Vincent Sheka, Sivaram Vipin P, Padikkala Jose
Department of Biochemistry, Amala Cancer Research Centre, Thrissur, Kerala, India.
Department of Biotechnology, St. Joseph's College, Thrissur, Kerala, India.
J Ayurveda Integr Med. 2016 Apr-Jun;7(2):78-82. doi: 10.1016/j.jaim.2016.06.003. Epub 2016 Jul 20.
Gmelina arborea (GA) is widely used in traditional medicine for treating a number of ailments including gastrointestinal tract disorders.
To evaluate the gastroprotective effect of GA stem bark against ethanol-induced gastric ulcer in Wistar rats.
All animals were fasted for 36 h and received GA extract 250 and 500 mg/kg body weight (bw), 1 h before the administration of ethanol. The animals received ranitidine 50 mg/kg bw which served as the standard. The rats were sacrificed after 4 h. Then, the injuries to the gastric mucosa were estimated through gross evaluation of ulcer lesions and histology. The antioxidant parameters such as level of lipid peroxidation, superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione peroxidase (GPx) in gastric tissue were also determined.
GA treatment at a dose of 500 mg/kg bw offered 91.98% inhibition of ulcer formation, which is higher than that of ranitidine. The ethanol treatment extensively increased lipid peroxidation and it was significantly (P < 0.01) reduced in GA-treated group that eventually helped to prevent free radical accumulation. The GA enhanced the gastric mucosal antioxidant system, as indicated by a dose-dependent increase in the level/activities of GSH, GPx, and SOD. GA also attenuated the severity of histological signs of cell damage. Further, GA extract showed in-vitro 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity with IC50 value of 124.39 μg/ml.
The results indicate that the gastroprotective effect of GA is probably related to its antioxidant activities that protect gastric mucosa against oxidative damage and antilipid peroxidative activity that maintain membrane integrity.
毛叶石梓（GA）在传统医学中被广泛用于治疗多种疾病，包括胃肠道疾病。
评估毛叶石梓茎皮对Wistar大鼠乙醇诱导的胃溃疡的胃保护作用。
所有动物禁食36小时，在给予乙醇前1小时接受250和500毫克/千克体重（bw）的GA提取物。动物接受50毫克/千克bw的雷尼替丁作为标准对照。4小时后处死大鼠。然后，通过对溃疡病变的大体评估和组织学来估计胃黏膜损伤。还测定了胃组织中的抗氧化参数，如脂质过氧化水平、超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）。
500毫克/千克bw剂量的GA处理对溃疡形成的抑制率为91.98%，高于雷尼替丁。乙醇处理显著增加了脂质过氧化，而GA处理组中脂质过氧化显著降低（P<0.01），最终有助于防止自由基积累。GA增强了胃黏膜抗氧化系统，表现为GSH、GPx和SOD水平/活性呈剂量依赖性增加。GA还减轻了细胞损伤的组织学体征的严重程度。此外，GA提取物显示出体外2,2-二苯基-1-苦基肼自由基清除活性，IC50值为124.39微克/毫升。
结果表明，GA的胃保护作用可能与其抗氧化活性有关，抗氧化活性可保护胃黏膜免受氧化损伤，以及抗脂质过氧化活性可维持膜完整性。
