Liu Jiping, Wang Junbang, Xu Jinfang, Xia Han, Wang Yue, Zhang Chunxue, Chen Wei, Zhang Huina, Liu Qi, Zhu Rong, Shi Yiqi, Shen Zihao, Xing Zhonggang, Gao Wenxia, Zhou Liqiang, Shao Jinliang, Shi Jiayu, Yang Xuejiao, Deng Yaxuan, Wu Li, Lin Quan, Zheng Changhong, Zhu Wenmin, Wang Congrong, Sun Yi E, Liu Zhongmin
Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China.
Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopedic Department of Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Cell Discov. 2021 Oct 26;7(1):99. doi: 10.1038/s41421-021-00329-3.
Large-scale COVID-19 vaccinations are currently underway in many countries in response to the COVID-19 pandemic. Here, we report, besides generation of neutralizing antibodies, consistent alterations in hemoglobin A1c, serum sodium and potassium levels, coagulation profiles, and renal functions in healthy volunteers after vaccination with an inactivated SARS-CoV-2 vaccine. Similar changes had also been reported in COVID-19 patients, suggesting that vaccination mimicked an infection. Single-cell mRNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) before and 28 days after the first inoculation also revealed consistent alterations in gene expression of many different immune cell types. Reduction of CD8 T cells and increase in classic monocyte contents were exemplary. Moreover, scRNA-seq revealed increased NF-κB signaling and reduced type I interferon responses, which were confirmed by biological assays and also had been reported to occur after SARS-CoV-2 infection with aggravating symptoms. Altogether, our study recommends additional caution when vaccinating people with pre-existing clinical conditions, including diabetes, electrolyte imbalances, renal dysfunction, and coagulation disorders.
为应对新冠疫情，许多国家目前正在大规模开展新冠疫苗接种工作。在此，我们报告，在接种灭活新冠病毒疫苗后，健康志愿者除产生中和抗体外，糖化血红蛋白、血清钠和钾水平、凝血指标及肾功能也出现了持续变化。新冠患者中也报告了类似变化，这表明接种疫苗模拟了一次感染。首次接种前及接种后28天对外周血单核细胞（PBMC）进行的单细胞mRNA测序（scRNA-seq）也显示，许多不同免疫细胞类型的基因表达出现了持续变化。典型的变化是CD8 T细胞减少，经典单核细胞含量增加。此外，scRNA-seq显示NF-κB信号增强，I型干扰素反应减弱，这已通过生物学检测得到证实，且据报道在新冠病毒感染后出现这些变化会加重症状。总之，我们的研究建议，对于患有糖尿病、电解质失衡、肾功能不全和凝血障碍等现有临床疾病的人群进行疫苗接种时应格外谨慎。
