Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
Department of Oral Health Sciences, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
Curr Issues Mol Biol. 2021 Oct 6;43(3):1451-1459. doi: 10.3390/cimb43030102.
Azithromycin displays immunomodulatory and anti-inflammatory effects in addition to broad-spectrum antimicrobial activity and is used to treat inflammatory diseases, including respiratory and odontogenic infections. Few studies have reported the effect of azithromycin therapy on bone remodeling processes. The aim of this study was to examine the effects of azithromycin on the osteogenic function of osteoblasts using osteoblast-like MC3T3-E1 cells. Cells were cultured in the presence of 0, 0.1, 1, and 10 µg/mL azithromycin, and cell proliferation and alkaline phosphatase (ALPase) activity were determined. In vitro mineralized nodule formation was detected with alizarin red staining. The expression of collagenous and non-collagenous bone matrix protein was determined using real-time PCR or enzyme-linked immunosorbent assays. In cells cultured with 10 µg/mL azithromycin, the ALPase activity and mineralized nodule formation decreased, while the type I collagen, bone sialoprotein, osteocalcin, and osteopontin mRNA expression as well as osteopontin and phosphorylated osteopontin levels increased. These results suggest that a high azithromycin concentration (10 µg/mL) suppresses mineralized nodule formation by decreasing ALPase activity and increasing osteopontin production, whereas low concentrations (≤l.0 µg/mL) have no effect on osteogenic function in osteoblastic MC3T3-E1 cells.
阿奇霉素除了具有广谱抗菌活性外，还具有免疫调节和抗炎作用，用于治疗炎症性疾病，包括呼吸道和牙源性感染。很少有研究报道阿奇霉素治疗对骨重塑过程的影响。本研究旨在使用成骨细胞样 MC3T3-E1 细胞研究阿奇霉素对成骨细胞成骨功能的影响。将细胞在 0、0.1、1 和 10μg/ml 阿奇霉素存在的情况下培养，并测定细胞增殖和碱性磷酸酶（ALPase）活性。通过茜素红染色检测体外矿化结节的形成。使用实时 PCR 或酶联免疫吸附测定法测定胶原蛋白和非胶原蛋白骨基质蛋白的表达。在培养有 10μg/ml 阿奇霉素的细胞中，ALPase 活性和矿化结节形成减少，而 I 型胶原、骨涎蛋白、骨钙素和骨桥蛋白 mRNA 表达以及骨桥蛋白和磷酸化骨桥蛋白水平增加。这些结果表明，高浓度阿奇霉素（10μg/ml）通过降低 ALPase 活性和增加骨桥蛋白的产生来抑制矿化结节的形成，而低浓度（≤1.0μg/ml）对成骨细胞样 MC3T3-E1 细胞的成骨功能没有影响。
