Kehelpannala Cheka, Rupasinghe Thusitha, Hennessy Thomas, Bradley David, Ebert Berit, Roessner Ute
School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia.
Sciex, 2 Gilda Ct, Mulgrave, VIC 3170, Australia.
Mol Omics. 2021 Dec 6;17(6):894-910. doi: 10.1039/d1mo00196e.
Lipids are a group of compounds with diverse structures that perform several important functions in plants. To unravel and better understand their functions, plant biologists have been using various lipidomic technologies including liquid-chromatography (LC)-mass spectrometry (MS). However, there are still significant challenges in LC-MS based plant lipidomics, which need to be addressed. In this review, we provide an overview of the key developments in LC-MS based lipidomic approaches to detect and identify plant lipids with emphasis on areas that can be further improved. Given that the cellular lipidome is estimated to contain hundreds of thousands of lipids, many of the lipid structures remain to be discovered. Furthermore, the plant lipidome is considered to be significantly more complex compared to that of mammals. Recent technical developments in mass spectrometry have made the detection of novel lipids possible; hence, approaches that can be used for plant lipid discovery are also discussed.
脂质是一组结构多样的化合物，在植物中发挥着多种重要功能。为了揭示并更好地理解其功能，植物生物学家一直在使用包括液相色谱（LC）-质谱（MS）在内的各种脂质组学技术。然而，基于LC-MS的植物脂质组学仍面临重大挑战，需要加以解决。在本综述中，我们概述了基于LC-MS的脂质组学方法在检测和鉴定植物脂质方面的关键进展，重点关注可进一步改进的领域。鉴于细胞脂质组估计包含数十万种脂质，许多脂质结构仍有待发现。此外，与哺乳动物的脂质组相比，植物脂质组被认为要复杂得多。质谱技术的最新进展使新型脂质的检测成为可能；因此，还讨论了可用于植物脂质发现的方法。
