Nosocomial Infections Unit, CNM, Carlos III Health Institute, Madrid, Spain.
Paediatric Infectious and Tropical Diseases Department, La Paz University Hospital and Translational Research Network in Paediatric Infectious Diseases (RITIP), Institute for Health Research IdiPAZ, Madrid, Spain.
Microbiologyopen. 2021 Oct;10(5):e1235. doi: 10.1002/mbo3.1235.
To characterize the Staphylococcus aureus strains colonizing healthy Spanish children.
Between March and July 2018, 1876 Spanish children younger than 14 years attending primary healthcare centers were recruited from rural and urban areas. Staphylococcus aureus colonization of the anterior nostrils was analyzed. MecA and mecC genes, antibiotic susceptibility, and genotyping according to the spa were determined in all strains, and the following toxins were examined: Panton-Valentine leucocidin (pvl), toxic shock syndrome toxin (tst), and exfoliative toxins (eta, etb, etd). Multilocus sequence typing (MLST) and staphylococcal cassette chromosome (SCCmec) typing were performed on methicillin-resistant Staphylococcus aureus (MRSA) strains, as well as pulsed-field gel electrophoresis (PFGE).
619 strains were isolated in 1876 children (33%), and 92% of them were sent for characterization to the Spanish National Centre of Microbiology (n = 572). Twenty (3.5%) of these strains were mecA-positive. Several spa types were detected among MRSA, being t002 the most frequently observed (30%), associating with SCCmec IVc. Among MSSA, 33% were positive for tst, while only 0.73% were positive for pvl. The 20 MRSA strains were negative for pvl, and 6 (30%) harbored the tst gene.
methicillin-resistant Staphylococcus aureus nasal colonization in Spanish children is rare, with t002 being the most observed spa type, associated with SCCmec IVc. None of the MRSA strains produced pvl, but up to 30% of S. aureus strains were positive for tst.
分析定植于健康西班牙儿童鼻腔的金黄色葡萄球菌（Staphylococcus aureus）菌株的特征。
2018 年 3 月至 7 月，研究人员招募了来自农村和城市地区的 1876 名 14 岁以下的西班牙儿童，分析其鼻腔金黄色葡萄球菌定植情况。所有菌株均检测 mecA 和 mecC 基因、抗生素敏感性以及 spa 基因分型，并检测 Panton-Valentine 白细胞毒素（pvl）、中毒性休克综合征毒素（tst）和表皮剥脱毒素（eta、etb、etd）。对耐甲氧西林金黄色葡萄球菌（MRSA）菌株进行多位点序列分型（MLST）和葡萄球菌盒染色体（SCCmec）分型，并进行脉冲场凝胶电泳（PFGE）。
在 1876 名儿童中分离出 619 株（33%），其中 92%（n=572）被送往西班牙国家微生物学中心进行特征分析。20 株（3.5%）为 mecA 阳性。在 MRSA 中检测到多种 spa 型，其中 t002 最为常见（30%），与 SCCmec IVc 相关。在 MSSA 中，33%的菌株 tst 阳性，而仅 0.73%的菌株 pvl 阳性。20 株 MRSA 菌株均未检测到 pvl，6 株（30%）携带 tst 基因。
在西班牙儿童中，耐甲氧西林金黄色葡萄球菌鼻腔定植较为罕见，t002 是最常见的 spa 型，与 SCCmec IVc 相关。MRSA 菌株均不产生 pvl，但多达 30%的金黄色葡萄球菌菌株 tst 阳性。
