社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）致儿童感染的基因组分析——一项西班牙多中心研究
Genomic analysis of community-associated methicillin-resistant (CA-MRSA) causing infections in children-a Spanish multicenter study.
作者信息
García-Cobos Silvia, Seco Alberca Natalia, Bravo-Queipo-de-Llano Blanca, Casquero-García Verónica, Ramírez de Arellano Eva, Calvo Cristina, Ruíz-Carrascoso Guillermo, Falces-Romero Iker, Larrosa Escartín Nieves, Viñado-Perez Belén, Martínez-López Miguel Ángel, Melendo Pérez Susana, Ruíz de Gopegui Enrique, Pérez Vázquez Santiago, Carrasco-Colom Jaime, Aracil García Belén, Pérez-Vázquez María, Méndez-Echevarría Ana, Oteo Iglesias Jesús
机构信息
Laboratorio de Referencia e Investigación en Resistencia a Antibióticos e Infecciones Relacionadas con la Asistencia Sanitaria, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain.
出版信息
Front Microbiol. 2025 May 9;16:1534840. doi: 10.3389/fmicb.2025.1534840. eCollection 2025.
OBJECTIVES
is one of the most common human pathogens causing skin and soft tissue infections (SSTIs) among children. This study investigated the molecular traits of community-associated methicillin-resistant (CA-MRSA) isolates causing infections in children in Spain.
METHODS
Antibiotic susceptibility testing and whole-genome sequencing were performed in 98 CA-MRSA isolates (4.2 median age, 52% males). The phylogenetic relationship, antibiotic resistance, virulence, and plasmid replicon genes content were investigated.
RESULTS
Resistance rates were found as follows: Erythromycin, 42.9%, which could be explained due to the presence of , , and genes; tobramycin, 27.5%, which could be explained due to the presence of and genes; tetracycline, 25.5%, which could be explained mainly due to the presence of genes; levofloxacin and moxifloxacin, 19.4%, which could be explained primarily due to the mutations in and genes; and gentamicin, 15.3%, which could be explained due to the presence of gene. The most prevalent lineage was ST8-IVc and t008. Most isolates were genetically diverse, except for three groups of isolates from the same hospital and one group of isolates from different hospitals. These had less than or equal to 5 allele differences by core-genome multilocus sequence typing (cgMLST) analysis or 0-6 core single-nucleotide polymorphisms (SNPs) by core-genome SNP-based analysis. Phage-encoded Panton-Valentine leukocidin (PVL) genes were found in 75.5% of the isolates. Other common virulence genes were related to adhesion ( and ), lipid degradation (), hemolysis (, , , and ), and tissue destruction ().
CONCLUSION
This study observed a high genetic diversity among CA-MRSA isolates causing community-acquired infections in children in Spain, with ST8-IVc as the most prevalent lineage. Nevertheless, genetic relatedness of some isolates from the same as well as different hospitals suggests the dissemination of CA-MRSA among children by contact.
目的
是引起儿童皮肤和软组织感染（SSTIs）的最常见人类病原体之一。本研究调查了西班牙引起儿童感染的社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）分离株的分子特征。
方法
对98株CA-MRSA分离株（中位年龄4.2岁，52%为男性）进行了药敏试验和全基因组测序。研究了系统发育关系、抗生素耐药性、毒力和质粒复制子基因含量。
结果
耐药率如下：红霉素为42.9%，这可能是由于存在、和基因所致；妥布霉素为27.5%，这可能是由于存在和基因所致；四环素为25.5%，这主要可能是由于存在基因所致；左氧氟沙星和莫西沙星为19.4%，这主要可能是由于和基因发生突变所致；庆大霉素为15.3%，这可能是由于存在基因所致。最常见的菌系是ST8-IVc和t008。除了来自同一家医院的三组分离株和来自不同医院的一组分离株外，大多数分离株在基因上是多样的。通过核心基因组多位点序列分型（cgMLST）分析，这些分离株的等位基因差异小于或等于5个，或者通过基于核心基因组单核苷酸多态性（SNP）的分析，其核心单核苷酸多态性为0-6个。在75.5%的分离株中发现了噬菌体编码的杀白细胞素（PVL）基因。其他常见的毒力基因与黏附（和）、脂质降解（）、溶血（、、和）以及组织破坏（）有关。
结论
本研究观察到在西班牙引起儿童社区获得性感染的CA-MRSA分离株之间存在高度的遗传多样性，其中ST8-IVc是最常见的菌系。然而，来自同一家医院以及不同医院的一些分离株的遗传相关性表明，CA-MRSA可通过接触在儿童中传播。
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