Department of Gastrointestinal Surgery, The Affiliated Zhongshan Hospital of Xiamen University; Institute of Gastrointestinal Oncology, Medical College of Xiamen University, Xiamen, China.
Medical Cosmetology, Xiamen Hospital of Traditional Chinese Medicine, Xiamen, China.
Bioengineered. 2021 Dec;12(2):9484-9495. doi: 10.1080/21655979.2021.1996014.
Linc00467 is a vital regulator in tumor progression. This study explores the molecular mechanisms of linc00467 in gastric cancer (GC). Linc00467 expression was obtained and analyzed in GC tissue through exploration in the cancer genome atlas database. Then, real-time quantitative polymerase chain reaction was used to detect linc000467 expression in GC cells. Cell functions were observed using cell counting Kit-8, Transwell assay, Western blotting to testify the proliferation, migration, invasion, and the relative expression of epidermal growth factor receptor (EGFR) in GC cells. Moreover, a dual-luciferase reporter gene assay was used to verify the relationship between linc00467 and miR-7-5p. Results showed that the expression of linc00467 was overexpressed in GC. Linc00467 silencing decreased the GC cell proliferation, migration, and invasion. With mRNA verification and combined previous research, linc00467 directly regulated the miR-7-5p expression and downstream EGFR expression. Inhibited miR-7-5p could restore cell function, EGFR expression of GC cells when linc00467 knockdown occurs. Altogether, linc00467 directly regulates the miR-7-5p and EGFR signaling pathway to promote GC proliferation, migration, and invasion.
Linc00467 是肿瘤进展的重要调节因子。本研究探讨了 linc00467 在胃癌（GC）中的分子机制。通过癌症基因组图谱数据库的探索，获得并分析了 GC 组织中的 linc00467 表达。然后，使用实时定量聚合酶链反应检测 GC 细胞中 linc000467 的表达。通过细胞计数试剂盒-8、Transwell 测定、Western blot 来观察 GC 细胞的功能，以验证细胞增殖、迁移、侵袭和表皮生长因子受体（EGFR）的相对表达。此外，还使用双荧光素酶报告基因测定来验证 linc00467 与 miR-7-5p 之间的关系。结果表明，linc00467 在 GC 中表达上调。沉默 linc00467 可降低 GC 细胞的增殖、迁移和侵袭。通过 mRNA 验证和结合先前的研究，linc00467 可直接调节 miR-7-5p 的表达和下游 EGFR 的表达。当 linc00467 敲低时，抑制 miR-7-5p 可恢复 GC 细胞的功能和 EGFR 表达。总之，linc00467 可通过直接调节 miR-7-5p 和 EGFR 信号通路来促进 GC 的增殖、迁移和侵袭。
