McMillan Christopher L D, Choo Jovin J Y, Idris Adi, Supramaniam Aroon, Modhiran Naphak, Amarilla Alberto A, Isaacs Ariel, Cheung Stacey T M, Liang Benjamin, Bielefeldt-Ohmann Helle, Azuar Armira, Acharya Dhruba, Kelly Gabrielle, Fernando Germain J P, Landsberg Michael J, Khromykh Alexander A, Watterson Daniel, Young Paul R, McMillan Nigel A J, Muller David A
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland 4072, Australia.
Menzies Health Institute Queensland, School of Pharmacy, Anatomy and Medical Sciences, Griffith University, Gold Coast, Queensland 4222, Australia.
Sci Adv. 2021 Oct 29;7(44):eabj8065. doi: 10.1126/sciadv.abj8065.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected more than 160 million people and resulted in more than 3.3 million deaths, and despite the availability of multiple vaccines, the world still faces many challenges with their rollout. Here, we use the high-density microarray patch (HD-MAP) to deliver a SARS-CoV-2 spike subunit vaccine directly to the skin. We show that the vaccine is thermostable on the patches, with patch delivery enhancing both cellular and antibody immune responses. Elicited antibodies potently neutralize clinically relevant isolates including the Alpha and Beta variants. Last, a single dose of HD-MAP–delivered spike provided complete protection from a lethal virus challenge in an ACE2-transgenic mouse model. Collectively, these data show that HD-MAP delivery of a SARS-CoV-2 vaccine was superior to traditional needle-and-syringe vaccination and may be a significant addition to the ongoing COVID-19 (coronavirus disease 2019) pandemic.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）已感染超过1.6亿人，并导致超过330万人死亡，尽管有多种疫苗可供使用，但全球在疫苗推广方面仍面临诸多挑战。在此，我们使用高密度微阵列贴片（HD-MAP）将SARS-CoV-2刺突亚基疫苗直接递送至皮肤。我们发现该疫苗在贴片上具有热稳定性，贴片给药可增强细胞免疫反应和抗体免疫反应。诱导产生的抗体能有效中和包括Alpha和Beta变体在内的临床相关毒株。最后，在ACE2转基因小鼠模型中，单剂量HD-MAP递送的刺突蛋白可提供完全保护，使其免受致死性病毒攻击。总体而言，这些数据表明，HD-MAP递送的SARS-CoV-2疫苗优于传统的针头注射疫苗，可能会为正在进行的2019冠状病毒病（COVID-19）大流行做出重大贡献。
