Department of Natural Medicine, School of Pharmacy, Fudan University, Shanghai 201203, PR China.
Wuxi School of Medicine, Jiangnan University, Wuxi 214122, PR China.
Fitoterapia. 2022 Jan;156:105068. doi: 10.1016/j.fitote.2021.105068. Epub 2021 Oct 29.
Three labdane-type [multisins A-C (1-3)], two guaiane-type [multisins D (4) and E (5)], and one eudesmane-type [multisin F (6)] previously undescribed terpenoids, together with 14 mono- (7-20) and seven dimeric- (21-27) known terpenoids, were isolated from the 90% MeOH extract of the whole plant of Chloranthus multistachys. Their structures and absolute configurations were determined by extensive spectroscopic methods and electronic circular dichroism (ECD) calculations. Compounds 4 and 5 are rare trinor-sesquiterpenes with a de-isopropyl guaiane skeleton, whereas compound 6 is a rearranged dinor-eudesmene featuring an uncommon octahydro-1H-indene ring system. Among the isolates, the dimeric lindenane sesquiterpenoid shizukaol C (25) exhibited the most potent (IC = 8.04 μM) anti-neuroinflammatory activity by inhibiting the nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated murine BV-2 microglial cells.
从多穗柯（Chloranthus multistachys）全株的 90%甲醇提取物中分离得到了三种从未被描述过的 labdane 型[倍半萜多辛 A-C（1-3）]、两种愈创木烷型[倍半萜多辛 D（4）和 E（5）]和一种 eudesmane 型[倍半萜多辛 F（6）]萜类化合物，以及 14 种单萜（7-20）和 7 种二萜（21-27）。通过广泛的光谱方法和电子圆二色性（ECD）计算确定了它们的结构和绝对构型。化合物 4 和 5 是罕见的 trinor-倍半萜，具有去异丙基愈创木烷骨架，而化合物 6 是一种重排的 dinor-艾里木烷，具有不常见的八氢-1H-茚环系统。在分离出的化合物中，二聚体 lindenane 倍半萜 shizukaol C（25）通过抑制脂多糖（LPS）刺激的小鼠 BV-2 小胶质细胞中一氧化氮（NO）的产生，表现出最强的（IC = 8.04 μM）抗炎活性。
