Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, 43124 Parma, Italy.
Department of Microbiology and Biochemistry of Dairy Products, Dairy Research Institute of Asturias, Spanish National Research Council (IPLA-CSIC), Paseo Río Linares sn 33300 Villaviciosa, Asturias, Spain; Functionality and Ecology of Beneficial Microbes (MicroHealth) Group, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Avda. Roma s/n, 33011 Oviedo, Asturias, Spain.
Syst Appl Microbiol. 2021 Nov;44(6):126273. doi: 10.1016/j.syapm.2021.126273. Epub 2021 Oct 19.
Ten Bifidobacterium strains, i.e., 6T3, 64T4, 79T10, 80T4, 81T8, 82T1, 82T10, 82T18, 82T24, and 82T25, were isolated from mantled guereza (Colobus guereza), Sumatran orangutan (Pongo abeli), silvery marmoset (Mico argentatus), golden lion tamarin (Leontopithecus rosalia), pied tamarin (Saguinus bicolor), and common pheasant (Phaisanus colchinus). Cells are Gram-positive, non-motile, non-sporulating, facultative anaerobic, and fructose 6-phosphate phosphoketolase-positive. Phylogenetic analyses based on the core genome sequences revealed that isolated strains exhibit close phylogenetic relatedness with Bifidobacterium genus members belonging to the Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium pullorum, and Bifidobacterium tissieri phylogenetic groups. Phenotypic characterization and genotyping based on the genome sequences clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species. Thus, B. phasiani sp. nov. (6T3 = LMG 32224 = DSM 112544), B. pongonis sp. nov. (64T4 = LMG 32281 = DSM 112547), B. saguinibicoloris sp. nov. (79T10 = LMG 32232 = DSM 112543), B. colobi sp. nov. (80T4 = LMG 32225 = DSM 112552), B. simiiventris sp. nov. (81T8 = LMG 32226 = DSM 112549), B. santillanense sp. nov. (82T1 = LMG 32284 = DSM 112550), B. miconis sp. nov. (82T10 = LMG 32282 = DSM 112551), B. amazonense sp. nov. (82T18 = LMG 32297 = DSM 112548), pluvialisilvae sp. nov. (82T24 = LMG 32229 = DSM 112545), and B. miconisargentati sp. nov. (82T25 = LMG 32283 = DSM 112546) are proposed as novel Bifidobacterium species.
从 mantle 猕猴（Colobus guereza）、苏门答腊猩猩（Pongo abeli）、银狨猴（Mico argentatus）、金狮面狨（Leontopithecus rosalia）、白脸卷尾猴（Saguinus bicolor）和普通雉鸡（Phaisanus colchicus）中分离出 10 株双歧杆菌菌株，即 6T3、64T4、79T10、80T4、81T8、82T1、82T10、82T18、82T24 和 82T25。细胞呈革兰氏阳性，非运动，非孢子形成，兼性厌氧，果糖 6-磷酸磷酸酮酶阳性。基于核心基因组序列的系统发育分析表明，分离株与双歧杆菌属成员密切相关，属于双歧杆菌双歧亚种、长双歧杆菌、Pullorum 双歧杆菌和 Tissieri 双歧杆菌系统发育群。基于基因组序列的表型特征和基因分型清楚地表明，这些菌株与迄今为止已识别的双歧杆菌物种的每种模式株都不同。因此，提出 Phasiani 双歧杆菌新种（6T3 = LMG 32224 = DSM 112544）、Pongonis 双歧杆菌新种（64T4 = LMG 32281 = DSM 112547）、Saguinibicoloris 双歧杆菌新种（79T10 = LMG 32232 = DSM 112543）、Colobi 双歧杆菌新种（80T4 = LMG 32225 = DSM 112552）、Simiventris 双歧杆菌新种（81T8 = LMG 32226 = DSM 112549）、Santillanense 双歧杆菌新种（82T1 = LMG 32284 = DSM 112550）、Miconis 双歧杆菌新种（82T10 = LMG 32282 = DSM 112551）、Amazonense 双歧杆菌新种（82T18 = LMG 32297 = DSM 112548）、Pluvialisilvae 双歧杆菌新种（82T24 = LMG 32229 = DSM 112545）和 Miconisargentati 双歧杆菌新种（82T25 = LMG 32283 = DSM 112546）。
