Laboratório de Patologia de Invertebrados (LPI), Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Avenida Esperança s/n, Campus Samambaia, GO, 74690-900, Goiânia, Brazil.
Laboratório de Nanosistemas e Dispositivos de Liberação de Fármacos (NanoSYS), Faculdade de Farmácia, UFG, Goiânia, GO, Brazil.
Appl Microbiol Biotechnol. 2021 Dec;105(23):8703-8714. doi: 10.1007/s00253-021-11644-w. Epub 2021 Oct 30.
Entomopathogenic fungi can achieve important innovative outcomes for integrated mosquito control especially of Aedes aegypti, the key vector of arboviruses to humans in the tropics and subtropics. This study sought to design and to develop a simple dissemination device to attract and to infect gravid A. aegypti adults with a granular formulation of the ascomycete Metarhizium humberi IP 46, and to validate this device in the laboratory as well as in semi-field and field conditions. Hydrogel (polyacrylamide potassium polyacrylate) was confirmed to be a suitable substitute for water used in the device that attracted gravid females under field conditions. Females laid eggs on black polyethylene terephthalate carpet fixed in the device that also proved to be a suitable substrate for a granular formulation of fungal microsclerotia and/or conidia. The plastic device (29.5 cm high) was divided into a lower closed compartment with a water reservoir and an upper, laterally open but covered compartment with continuously hydrated gel and the fungal formulation attached to the carpet. The uppermost compartment permitted free circulation of mosquito adults. The device attracted both male and female A. aegypti. The fungal formulations of IP 46 propagules tested in the device were effective against adults in laboratory, semi-field, and field settings. Findings in the laboratory, semi-field, and especially in field conditions strengthen the value and utility of this innovative device for focal applications of a mycoinsecticide against this important mosquito vector.Key points• Low-cost and simple disseminating device for focal control of Aedes aegypti.• Granulized Metarhizium humberi IP 46 and hydrogel yield extended control.• Findings in field tests strengthen benefit of the device for focal application.
昆虫病原真菌可以为综合蚊虫控制（尤其是对热带和亚热带地区人类的虫媒病毒的主要载体埃及伊蚊）取得重要的创新成果。本研究旨在设计和开发一种简单的传播装置，以吸引并感染带菌的埃及伊蚊成虫，使用的是粗糙脉孢菌 IP 46 的颗粒制剂，并在实验室、半野外和野外条件下对该装置进行验证。水凝胶（聚丙烯酸钾聚丙烯酸酯）被证实是一种适合替代用于吸引野外条件下带菌雌性的装置中的水的替代品。雌性将卵产在固定在装置中的黑色聚对苯二甲酸乙二醇酯地毯上，该装置也被证明是真菌微菌核和/或分生孢子颗粒制剂的合适基质。该塑料装置（高 29.5 厘米）分为下部封闭的水箱和上部横向开放但覆盖的部分，带有连续水合的凝胶和附着在地毯上的真菌制剂。最高部分允许成年蚊子自由循环。该装置吸引了埃及伊蚊的雄性和雌性。在装置中测试的 IP 46 分生孢子真菌制剂对实验室、半野外和野外环境中的成虫均有效。实验室、半野外和特别是野外条件下的发现增强了这种创新装置对这种重要蚊子传播媒介的点状应用的价值和实用性。
关键点
• 针对埃及伊蚊的点状控制的低成本和简单传播装置。
• 颗粒化粗糙脉孢菌 IP 46 和水凝胶可延长控制效果。
• 野外测试结果增强了该装置点状应用的益处。
