Rodrigues Juscelino, Catão Alaine Maria Lopes, Dos Santos Amanda Soares, Paixão Flávia Regina Santos, Santos Thainá Rodrigues, Martinez Juan Mercado, Marreto Ricardo Neves, Mascarin Gabriel Moura, Fernandes Éverton Kort Kamp, Humber Richard Alan, Luz Christian
Laboratório de Patologia de Invertebrados (LPI), Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Avenida Esperança s/n, Campus Samambaia, Goiânia, GO, 74690-900, Brazil.
Laboratório de Nanosistemas e Dispositivos de Liberação de Fármacos (NanoSYS), Faculdade de Farmácia, UFG, Goiânia, GO, Brazil.
Appl Microbiol Biotechnol. 2021 Apr;105(7):2725-2736. doi: 10.1007/s00253-021-11157-6. Epub 2021 Mar 21.
The impact of ambient relative humidity (RH) on conidial production of Metarhizium humberi IP 46 microsclerotia (MS) formulated in pellets or granules was investigated, and a promising granular formulation was tested against Aedes aegypti adults to confirm its efficacy. Microcrystalline cellulose (MC) and diatomaceous earth (DE) or a combination of vermiculite (VE), DE and silicon dioxide (SD) were tested as carriers in granular formulations containing MS. A range of 93-96.5% RH was critical for fungal development, and at least 96.5-98.5% RH was required for high conidial production on pellets or granules. Conidial production was clearly higher on pellets and granules prepared with VE than MC as the main carrier. VE granules containing MS were highly active against A. aegypti adults. Most mosquitoes were killed within 6 days after treatment regardless of the exposure time of adults to the formulation (1 min-24 h) or ambient humidity (75 or >98%). Production of conidia on dead adults varied between 7.3 × 10 and 2.2 × 10 conidia/individual, when exposed to MS granules for 12 h and 1 min, respectively. Granular formulations containing VE as the main carrier and MS as the active ingredient of M. humberi have strong potential for use against A. aegypti. KEY POINTS: • High conidial production on granular microsclerotial formulations at >96.5% RH • Vermiculite is more appropriate as a carrier than microcrystalline cellulose • Granules with IP 46 microsclerotia are highly active against Aedes aegypti adults.
研究了环境相对湿度（RH）对以丸剂或颗粒剂形式配制的亨氏绿僵菌IP 46微菌核（MS）分生孢子产生的影响，并对一种有前景的颗粒剂配方进行了埃及伊蚊成虫试验以确认其功效。微晶纤维素（MC）、硅藻土（DE）或蛭石（VE）、DE和二氧化硅（SD）的组合作为含有MS的颗粒剂配方中的载体进行了测试。93-96.5%的RH范围对真菌发育至关重要，在丸剂或颗粒剂上产生高产量分生孢子至少需要96.5-98.5%的RH。以VE为主要载体而非MC制备的丸剂和颗粒剂上的分生孢子产量明显更高。含有MS的VE颗粒对埃及伊蚊成虫具有高活性。无论成虫对配方的暴露时间（1分钟至24小时）或环境湿度（75%或>98%）如何，大多数蚊子在处理后6天内死亡。当分别暴露于MS颗粒12小时和1分钟时，死蚊上的分生孢子产量在7.3×10至2.2×10个分生孢子/个体之间变化。以VE为主要载体、MS为亨氏绿僵菌活性成分的颗粒剂配方在防治埃及伊蚊方面具有很大的应用潜力。要点：• 在>96.5%RH下颗粒微菌核配方上分生孢子产量高 • 蛭石比微晶纤维素更适合作为载体 • 含有IP 46微菌核的颗粒对埃及伊蚊成虫具有高活性
