School of Chemistry, College of Science, University of Tehran, Tehran, Iran.
J Biol Inorg Chem. 2022 Feb;27(1):81-87. doi: 10.1007/s00775-021-01912-5. Epub 2021 Oct 30.
Antibacterial materials are an essential part of modern life and many efforts have been made to find a new and effective type of them. In this study, chlorhexidine (CHX) was loaded on Cu-BTC metal-organic framework (MOF), that both of them are known to have antibacterial properties. The antibacterial properties of Cu-BTC, CHX and CHX@Cu-BTC were investigated against Gram-positive and Gram-negative bacteria. Agar well-diffusion method and MIC test showed that CHX@Cu-BTC has high antibacterial activity. Characterization methods, such as FT-IR, XRD, N adsorption-desorption isotherm, TGA, SEM, EDX, TEM and zeta potential, were employed to characterize their structures. Cu-BTC MOF nanoparticles were synthesized and used as nanoporous carriers for chlorhexidine. The loading was about 10%, which was absorbed into the pores. Antibacterial activity was investigated against Gram-negative and Gram-positive bacteria by Agar well diffusion method and MIC (minimal inhibitory concentration) assay. The CHX@Cu-BTC had synergistic antibacterial activity of Cu-BTC and chlorhexidine.
抗菌材料是现代生活的重要组成部分，人们已经做出了许多努力来寻找一种新型且有效的抗菌材料。在这项研究中，将洗必泰（CHX）负载到具有抗菌性能的铜基金属有机骨架（MOF）材料 Cu-BTC 上。研究了 Cu-BTC、CHX 和 CHX@Cu-BTC 对革兰氏阳性菌和革兰氏阴性菌的抗菌性能。琼脂扩散法和 MIC 试验表明 CHX@Cu-BTC 具有很高的抗菌活性。采用 FT-IR、XRD、N 吸附-解吸等温线、TGA、SEM、EDX、TEM 和zeta 电位等表征方法对其结构进行了表征。合成了 Cu-BTC MOF 纳米粒子，并将其用作洗必泰的纳米多孔载体。负载量约为 10%，被吸附到孔中。采用琼脂扩散法和 MIC（最小抑菌浓度）试验研究了 CHX@Cu-BTC 对革兰氏阴性菌和革兰氏阳性菌的抗菌活性。CHX@Cu-BTC 具有铜基金属有机骨架（MOF）和洗必泰的协同抗菌活性。
