Chi Cheng, Xu Xuechen, Wang Mengqi, Zhang Hui, Fang Pingping, Zhou Jie, Xia Xiaojian, Shi Kai, Zhou Yanhong, Yu Jingquan
Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, P.R. China.
Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, 866 Yuhangtang Road, Hangzhou, 310058, P.R. China.
Hortic Res. 2021 Nov 1;8(1):237. doi: 10.1038/s41438-021-00668-y.
Strigolactones are carotenoid-derived phytohormones that impact plant growth and development in diverse ways. However, the roles of strigolactones in the responses to temperature stresses are largely unknown. Here, we demonstrated that strigolactone biosynthesis is induced in tomato (Solanum lycopersicum) by heat and cold stresses. Compromised strigolactone biosynthesis or signaling negatively affected heat and cold tolerance, while application of the synthetic strigolactone analog GR24 enhanced heat and cold tolerance. Strigolactone-mediated heat and cold tolerance was associated with the induction of abscisic acid (ABA), heat shock protein 70 (HSP70) accumulation, C-REPEAT BINDING FACTOR 1 (CBF1) transcription, and antioxidant enzyme activity. Importantly, a deficiency in ABA biosynthesis compromised the GR24 effects on heat and cold stresses and abolished the GR24-induced transcription of HSP70, CBF1, and antioxidant-related genes. These results support that strigolactones positively regulate tomato heat and cold tolerance and that they do so at least partially by the induction of CBFs and HSPs and the antioxidant response in an ABA-dependent manner.
独脚金内酯是一类由类胡萝卜素衍生而来的植物激素，它们以多种方式影响植物的生长和发育。然而，独脚金内酯在植物应对温度胁迫方面的作用在很大程度上仍不清楚。在此，我们证明了热胁迫和冷胁迫可诱导番茄（Solanum lycopersicum）中独脚金内酯的生物合成。独脚金内酯生物合成或信号转导受损会对耐热性和耐寒性产生负面影响，而施用合成独脚金内酯类似物GR24则可增强耐热性和耐寒性。独脚金内酯介导的耐热性和耐寒性与脱落酸（ABA）的诱导、热激蛋白70（HSP70）的积累、C-重复结合因子1（CBF1）的转录以及抗氧化酶活性有关。重要的是，ABA生物合成缺陷会削弱GR24对热胁迫和冷胁迫的影响，并消除GR24诱导的HSP70、CBF1和抗氧化相关基因的转录。这些结果表明，独脚金内酯正向调控番茄的耐热性和耐寒性，并且它们至少部分是通过以ABA依赖的方式诱导CBF和HSP以及抗氧化反应来实现的。
