Hu Yanping, Zhang Tingting, Liu Ying, Li Yuxin, Wang Min, Zhu Baibi, Liao Daolong, Yun Tianhai, Huang Wenfeng, Zhang Wen, Zhou Yang
Key Laboratory of Vegetable Biology of Hainan Province, Hainan Vegetable Breeding Engineering Technology Research Center, The Institute of Vegetables, Hainan Academy of Agricultural Sciences, Haikou, China.
Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou, China.
Front Genet. 2021 Oct 14;12:753953. doi: 10.3389/fgene.2021.753953. eCollection 2021.
Pumpkin () is an important cucurbit vegetable crop that has strong resistance to abiotic stress. While heat shock protein 20 (HSP20) has been implicated in vegetable response to heat stress, little is known regarding activity of HSP20 family proteins in . Here, we performed a comprehensive genome-wide analysis to identify and characterize the functional dynamics of the () gene family. A total of 33 genes distributed across 13 chromosomes were identified from the pumpkin genome. Our phylogenetic analysis determined that the CmoHSP20 proteins fell into nine distinct subfamilies, a division supported by the conserved motif composition and gene structure analyses. Segmental duplication events were shown to play a key role in expansion of the gene family. Synteny analysis revealed that 19 and 18 genes were collinear with those in the cucumber and melon genomes, respectively. Furthermore, the expression levels of pumpkin genes were differentially induced by heat stress. The transcript level of , and were down-regulated by heat stress, while , , , , and were up-regulated by heat stress, which could be used as heat tolerance candidate genes. Overall, these findings contribute to our understanding of vegetable HSP20 family genes and provide valuable information that can be used to breed heat stress resistance in cucurbit vegetable crops.
南瓜（）是一种重要的葫芦科蔬菜作物，对非生物胁迫具有较强的抗性。虽然热休克蛋白20（HSP20）与蔬菜对热胁迫的反应有关，但关于南瓜中HSP20家族蛋白的活性却知之甚少。在此，我们进行了全面的全基因组分析，以鉴定和表征南瓜（）基因家族的功能动态。从南瓜基因组中鉴定出总共33个分布在13条染色体上的CmoHSP20基因。我们的系统发育分析确定，CmoHSP20蛋白分为九个不同的亚家族，这一划分得到了保守基序组成和基因结构分析的支持。片段重复事件在CmoHSP20基因家族的扩展中起关键作用。共线性分析表明，分别有19个和18个CmoHSP20基因与黄瓜和甜瓜基因组中的基因共线。此外，南瓜CmoHSP20基因的表达水平受到热胁迫的差异诱导。CmoHSP20-1、CmoHSP20-2和CmoHSP20-3的转录水平受到热胁迫的下调，而CmoHSP20-5、CmoHSP20-6、CmoHSP20-7、CmoHSP20-8、CmoHSP20-9和CmoHSP20-10受到热胁迫的上调，这些基因可作为耐热候选基因。总体而言，这些发现有助于我们理解蔬菜HSP20家族基因，并为培育葫芦科蔬菜作物的耐热性提供有价值的信息。
