Rahma Nefranindy, Wurlina Wurlina, Madyawati Sri Pantja, Utomo Budi, Hernawati Tatik, Safitri Erma
Practicing Veterinarian,Surabaya, Indonesia.
Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Open Vet J. 2021 Jul-Sep;11(3):401-406. doi: 10.5455/OVJ.2021.v11.i3.11. Epub 2021 Aug 10.
Malnutrition can cause an increase in oxidative stress as it triggers the expression of heat shock protein70 (HSP70), a chaperon molecule that is needed to repair damaged cells within optimal levels. Honey is a source of feed that can stimulate HSP70 expression, which can be given to the malnourished in the animal trial.
The purpose of this study was to prove that Kaliandra honey can improve testosterone levels, diameter, and epithelial thickness of the seminiferous tubule of rat testes () due to malnutrition through stimulation of HSP70, which is expressed immunohistochemically.
This study used 40 male rats, which were divided into four treatment groups: T0 (negative control): normal rats and not given honey; T1 (positive control): malnourished rats and not given honey; T2 (treatment 2): malnourished rats and given 30% Kaliandra honey (v/v) for 10 days; T3 (treatment 3), malnourished rats and given 50% Kaliandra honey (v/v) for 10 days. The condition of malnutrition is carried out by fasting the feed for five consecutive days resulting in damage to the male reproductive organs, especially the testes.
The results showed that Kaliandra honey at a dose of 50% (v/v) had a significant effect in improving testosterone levels, diameter, and epithelial thickness of seminiferous tubule of malnourished male rats through stimulation of HSP70 expression. The HSP70 expression scores by IHC at T0, T1, T2, and T3 were 0.15 ± 0.5, 3.15 ± 0.4, 2.95 ± 0.35, and 1.75 ± 0.15, sequentially. enzyme-linked immunosorbent assay indirect testosterone levels at T0, T1, T2, and T3 (in μg/dl) were 36.39 ± 0.35, 6.12 ± 0.51, 7.45 ± 0.15, 25.27 ± 0.63, sequentially. The diameter and epithelial thickness of the seminiferous tubule of the testes (in μm) in the four treatments T0, T1, T2, and T3 were 362.40 ± 4.71, 248.46 ± 3.90, 255.22 ± 2.34, 318.37 ± 4.23 and 117.60 ± 11.30, 3.86 ± 1.57, 9.72 ± 3.96, 29.84 ± 4.02 sequentially.
The conclusion of the study showed that Kaliandra honey at a dose of 50% (v/v) had a significant effect in improving testosterone levels, diameter, and epithelial thickness of the seminiferous tubule of malnourished rats through stimulation of HSP70, although not significantly the same as negative control (T0).
营养不良会导致氧化应激增加，因为它会触发热休克蛋白70（HSP70）的表达，HSP70是一种伴侣分子，在最佳水平下修复受损细胞时是必需的。蜂蜜是一种能刺激HSP70表达的饲料来源，可在动物试验中给予营养不良的动物。
本研究的目的是证明卡利安德拉蜂蜜可通过刺激免疫组化表达的HSP70，改善因营养不良导致大鼠睾丸（）生精小管的睾酮水平、直径和上皮厚度。
本研究使用40只雄性大鼠，分为四个治疗组：T0（阴性对照）：正常大鼠，未给予蜂蜜；T1（阳性对照）：营养不良大鼠，未给予蜂蜜；T2（治疗2）：营养不良大鼠，给予30%卡利安德拉蜂蜜（v/v），持续10天；T3（治疗3），营养不良大鼠，给予50%卡利安德拉蜂蜜（v/v），持续10天。通过连续五天禁食饲料造成营养不良状态，导致雄性生殖器官尤其是睾丸受损。
结果表明，50%（v/v）剂量的卡利安德拉蜂蜜通过刺激HSP70表达，对改善营养不良雄性大鼠的睾酮水平、生精小管直径和上皮厚度有显著作用。T0、T1、T2和T3组通过免疫组化检测的HSP70表达评分依次为0.15±0.5、3.15±0.4、2.95±0.35和1.75±0.15。T0、T1、T2和T3组间接酶联免疫吸附测定睾酮水平（μg/dl）依次为36.39±0.35、6.12±0.51、7.45±0.15、25.27±0.63。四个处理组T0、T1、T2和T3睾丸生精小管的直径和上皮厚度（μm）依次为362.40±4.71、248.46±3.90、255.22±2.34、318.37±4.23和117.60±11.30、3.86±1.57、9.72±3.96、29.84±4.02。
研究结论表明，50%（v/v）剂量的卡利安德拉蜂蜜通过刺激HSP70，对改善营养不良大鼠的睾酮水平、生精小管直径和上皮厚度有显著作用，尽管与阴性对照（T0）相比差异不显著。
