Liang Jing, Zhou Hui, Huang Xiang-Qi, Liu Yan-Fei, Zhang Lei, He Dan, Cui Yongmei, Guo Jinrui, Hu Kunpeng, Wu Chong
Department of Pathology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Department of General Surgery, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Front Mol Biosci. 2021 Oct 13;8:742953. doi: 10.3389/fmolb.2021.742953. eCollection 2021.
Intrahepatic cholangiocarcinoma (iCCA) is the second most common cancer in liver, with a high recurrence rate after surgery. Recently, we identified a CD11b-CD169-based myeloid response score (MRS), which showed remarkable prognostic potential in hepatocellular carcinoma (HCC). Here, we aimed to verify the prognostic value of the MRS in iCCA and establish an MRS-based nomogram to predict the postoperative prognosis of iCCA patients. From April 2005 to March 2017, a total of 84 patients from the Third Affiliated Hospital of Sun Yat-sen University were enrolled. Preoperative clinical information and surgical specimens of enrolled patients were collected. Among these, tissues from 75 patients passed the clinical data quality control and the staining quality control. The protein expression of CD11b and CD169 in iCCA samples were detected by immunohistochemistry (IHC). Kaplan-Meier analysis and receiver operating characteristic (ROC) curves revealed that the MRS had a high discriminatory ability for predicting the time to recurrence (TTR) of iCCA patients after surgery. Three independent risk factors selected by a Cox proportional hazards regression analysis, namely, the MRS, the tumor size and the status of vascular invasion, were included to construct a nomogram to predict the recurrence of iCCA after resection surgery. ROC curves, calibration analysis and decision curve analysis (DCA) suggested that this nomogram had notable discriminatory power, stability and clinical usefulness in predicting the postoperative recurrence. Together, we explored the prognostic value of the MRS in iCCA, and constructed an MRS-based nomogram which may help to predict postoperative recurrence and aid clinical decisions for iCCA patients.
肝内胆管癌（iCCA）是肝脏中第二常见的癌症，术后复发率高。最近，我们确定了一种基于CD11b - CD169的髓系反应评分（MRS），其在肝细胞癌（HCC）中显示出显著的预后潜力。在此，我们旨在验证MRS在iCCA中的预后价值，并建立基于MRS的列线图以预测iCCA患者的术后预后。2005年4月至2017年3月，中山大学附属第三医院共纳入84例患者。收集纳入患者的术前临床信息和手术标本。其中，75例患者的组织通过了临床数据质量控制和染色质量控制。通过免疫组织化学（IHC）检测iCCA样本中CD11b和CD169的蛋白表达。Kaplan - Meier分析和受试者工作特征（ROC）曲线显示，MRS对预测iCCA患者术后复发时间（TTR）具有较高的鉴别能力。通过Cox比例风险回归分析选择的三个独立危险因素，即MRS、肿瘤大小和血管侵犯状态，被纳入构建预测iCCA切除术后复发的列线图。ROC曲线、校准分析和决策曲线分析（DCA）表明，该列线图在预测术后复发方面具有显著的鉴别力、稳定性和临床实用性。我们共同探讨了MRS在iCCA中的预后价值，并构建了基于MRS的列线图，这可能有助于预测术后复发并辅助iCCA患者的临床决策。
