Zhang Hang, Wang Minjuan, Jia Junpeng, Zhao Jiayi, Radebe Stoffel Matjeke, Yu Qinghua
Ministry of Education (MOE) Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Front Vet Sci. 2021 Oct 15;8:740424. doi: 10.3389/fvets.2021.740424. eCollection 2021.
Intensive farming is prone to induce large-scale outbreaks of infectious diseases, with increasing use of antibiotics, which deviate from the demand of organic farming. The high mortality rate of chickens infected with caused huge economic losses; therefore, the promising safe prevention and treatment measures of are in urgent need, such as probiotics. Probiotics are becoming an ideal alternative treatment option besides antibiotics, but the effective chicken probiotic strains with clear protective mechanism against remain unclear. In this study, we found YQH2 was effective in preventing infection in chickens. induced the loss of body weight, and liver and intestinal morphology damage. The inflammatory factor levels increased and intestinal proliferation inhibited. However, after treatment with YQH2, broilers grew normally, the pathological changes of liver and intestine were reduced, and the colonization of in the intestine was improved. Not only that, the length of villi and the depth of crypts were relatively normal, and the levels of inflammatory factors such as IL-1β, TNF-α, and IL-8 were reduced. The number of PCNA cells of YQH2 returned to normal under the action of infection, which was conducive to the normal proliferation of intestinal epithelial cells. The protective effect of YQH2 may be due to the attribution to the activation of hypoxia and then induced the proliferation of intestinal stem cells to repair the damage of intestinal mucosa under infection. This study demonstrated that YQH2 was effective in preventing infection, which could be further used in the chicken health breeding.
集约化养殖容易引发大规模传染病爆发，随着抗生素使用的增加，这偏离了有机养殖的需求。感染[病原体名称未明确]的鸡死亡率高，造成了巨大的经济损失；因此，迫切需要有前景的安全预防和治疗措施，比如益生菌。益生菌正成为除抗生素之外理想的替代治疗选择，但针对[病原体名称未明确]具有明确保护机制的有效鸡用益生菌菌株仍不清楚。在本研究中，我们发现YQH2在预防鸡感染[病原体名称未明确]方面有效。[病原体名称未明确]感染导致体重减轻、肝脏和肠道形态损伤。炎症因子水平升高且肠道增殖受到抑制。然而，用YQH2处理后，肉鸡生长正常，肝脏和肠道的病理变化减轻，[病原体名称未明确]在肠道中的定植情况得到改善。不仅如此，绒毛长度和隐窝深度相对正常，白细胞介素 - 1β、肿瘤坏死因子 - α和白细胞介素 - 8等炎症因子水平降低。在[病原体名称未明确]感染作用下，YQH2处理组的增殖细胞核抗原（PCNA）细胞数量恢复正常，这有利于肠道上皮细胞的正常增殖。YQH2的保护作用可能归因于激活缺氧状态，进而诱导肠道干细胞增殖以修复[病原体名称未明确]感染下的肠道黏膜损伤。本研究表明YQH2在预防[病原体名称未明确]感染方面有效，可进一步用于鸡的健康养殖。
