Naef Hamdan, Alhussen Mohammad Abed, Vatnikov Yury Anatolyevich, Cheskidova Liliya Valeryevna, Semenova Valentina Ivanovna, Parshin Pavel Andreevich, Alsalh Mohammad Almohammad
Department of Veterinary Medicine, Peoples' Friendship University of Russia (RUDN University), Moscow, Russia.
Federal State Budgetary Scientific Institution, Russian Ministry of Agriculture, All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy, Farong, Russia.
J Adv Vet Anim Res. 2021 Jun 2;8(3):355-360. doi: 10.5455/javar.2021.h522. eCollection 2021 Sep.
The research was conducted to study the effect of a complex antimicrobial drug with an anti-inflammatory effect and an antimicrobial drug with an immunostimulating effect on the parameters of nonspecific resistance in calves.
Two groups ( = 5 each) of sick calves with respiratory pathology were selected for this study. For the treatment of the first experimental group, a complex antimicrobial drug Sulfetrisan was used. The second experimental group of the calves was intramuscularly injected with the experimental drug gentaaminoseleferon (GIA). To assess the cellular component of immunity in the blood before and after treatment, the number of white blood cells, T-lymphocytes, B-lymphocytes, phagocytic activity of leukocytes, phagocytic number, and phagocytic index (PhI) were determined. In addition, for assessing the humoral component, serum complement activity (SCA), serum lysozyme activity, serum bactericidal activity (SBA), circulating immune complexes (CIC), and total immunoglobulins (total Ig) were measured. The results were compared with the baseline parameters of healthy calves of the control group.
When studying the parameters of the humoral and cellular components of nonspecific resistance, it was found that in sick animals, compared with healthy ones, respiratory pathology was accompanied by an imbalance in the immune system. In the process of recovery in animals of the experimental groups under the effect of the drugs, positive changes occurred. However, many of the studied parameters did not reach the values of healthy animals. In the group of calves that received GIA, compared with the calves given Sulfetrisan, a significant increase in PhI ( < 0.05), SBA ( < 0.006), SCA ( < 0.05), total Ig ( < 0.0005), and CIC ( < 0.05) was observed, which indicated an increase in natural resistance due to the immunostimulating action.
The use of GIA in sick animals added to an increase in the general nonspecific cellular and humoral resistance of calves, which made it possible to increase therapeutic efficacy and shorten their recovery time.
本研究旨在探讨一种具有抗炎作用的复合抗菌药物和一种具有免疫刺激作用的抗菌药物对犊牛非特异性抵抗力参数的影响。
本研究选取两组（每组n = 5）患有呼吸道疾病的患病犊牛。第一实验组采用复合抗菌药物磺胺三嗪进行治疗。第二实验组的犊牛肌肉注射实验药物庆大霉素硒铁蛋白（GIA）。为评估治疗前后血液中免疫细胞成分，测定白细胞、T淋巴细胞、B淋巴细胞数量、白细胞吞噬活性、吞噬数和吞噬指数（PhI）。此外，为评估体液成分，检测血清补体活性（SCA）、血清溶菌酶活性、血清杀菌活性（SBA）、循环免疫复合物（CIC）和总免疫球蛋白（总Ig）。将结果与对照组健康犊牛的基线参数进行比较。
在研究非特异性抵抗力的体液和细胞成分参数时发现，与健康动物相比，患病动物的呼吸道疾病伴有免疫系统失衡。在实验组动物药物作用下的恢复过程中，出现了积极变化。然而，许多研究参数未达到健康动物的值。在接受GIA的犊牛组中，与给予磺胺三嗪的犊牛相比，观察到PhI（P < 0.05）、SBA（P < 0.006）、SCA（P < 0.05）、总Ig（P < 0.0005）和CIC（P < 0.05）显著增加，这表明由于免疫刺激作用，自然抵抗力增强。
在患病动物中使用GIA增加了犊牛总体非特异性细胞和体液抵抗力，从而提高了治疗效果并缩短了恢复时间。
