Department of Animal Sciences and Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58108,USA.
Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA.
J Anim Sci. 2021 Nov 1;99(11). doi: 10.1093/jas/skab201.
Multiparous Rambouillet ewes (n = 32) were allocated in a completely randomized design to determine if rumen-protected L-arginine (RP-Arg) supplementation during mid- and late gestation would 1) alter maternal carotid artery hemodynamics and 2) affect circulating amino acids associated with arginine metabolism in dams from day 54 of gestation to parturition and in their offspring from birth to 54 d of age. Ewes were assigned to one of three treatments from day 54 ± 3.9 to parturition: control (CON; 100% nutrient requirements), restricted (RES; 60% of CON), and RES plus 180 mg RP-Arg•kg BW-1•d1 (RES-ARG). Ewes were penned individually in a temperature-controlled facility. Carotid artery hemodynamics was measured via Doppler ultrasound at day 50 and 130 of gestation. Maternal serum was collected at day 54 and 138 of gestation and at parturition. At parturition, lambs were immediately removed from their dams and reared independently. Lamb serum samples were collected at birth and 1, 3, 7, 33, and 54 d of age. Pulsatility index was the only hemodynamic measurement altered by dietary treatment, where day 130 measurements were greater (P ≤ 0.04) for RES and RES-ARG compared with CON. The change in pulsatility index was greater (P < 0.01) for RES compared with CON but tended to be intermediate (P ≥ 0.12) for RES-ARG. Maternal serum Arg, Cit, and Asp at day 138 were greater (P < 0.01) for CON compared with RES and RES-ARG; serum Orn at day 138 was greater (P = 0.04) for CON compared with RES. Maternal serum Cit at parturition was greater (P ≤ 0.03) for CON and RES-ARG compared with RES. Offspring serum Arg was affected by a maternal treatment by day of age interaction (P = 0.03), where at day 3, CON and RES-ARG had greater (P ≤ 0.03) serum Arg concentrations than RES, and at day 54, RES-ARG was greater than (P = 0.002) CON and RES was intermediate and did not differ from (P ≥ 0.09) CON and RES-ARG. Offspring serum Orn and Cit were less (P ≤ 0.03) for RES and RES-ARG compared with CON. Results indicate that distal tissue blood perfusion decreased due to maternal RES, and RES-ARG was able to improve perfusion but not to the level of CON ewes. Further, maternal RP-Arg altered offspring Arg and related amino acid concentrations during the postnatal period.
多胎罗姆尼母羊（n=32）被完全随机分配，以确定在妊娠中期和晚期补充保护型 L-精氨酸（RP-Arg）是否会 1）改变母体颈动脉血液动力学，2）影响妊娠第 54 天至分娩时母羊和出生后 54 天的后代中与精氨酸代谢相关的循环氨基酸。从第 54 ± 3.9 天到分娩，母羊被分配到三种处理之一：对照组（CON；100%营养需求）、限制组（RES；60%的 CON）和 RES 加 180mg RP-Arg•kg BW-1•d1（RES-ARG）。母羊被单独关在一个温度控制的设施中。在妊娠第 50 天和 130 天通过多普勒超声测量颈动脉血液动力学。在妊娠第 54 天、138 天和分娩时采集母血清。分娩时，羔羊立即从母羊身上移开并独立饲养。在出生时和 1、3、7、33 和 54 天时采集羔羊血清样本。脉动指数是唯一受饮食处理改变的血液动力学测量值，RES 和 RES-ARG 的第 130 天测量值更高（P≤0.04）。与 CON 相比，RES 的脉动指数变化更大（P＜0.01），但 RES-ARG 的趋势是中间值（P≥0.12）。与 RES 和 RES-ARG 相比，妊娠第 138 天母血清 Arg、Cit 和 Asp 更高（P＜0.01）；妊娠第 138 天母血清 Orn 更高（P=0.04）。分娩时，CON 和 RES-ARG 的母血清 Cit 高于 RES（P≤0.03）。与 RES 相比，仔羊血清 Arg 受到母羊处理与日龄相互作用的影响（P=0.03），第 3 天，CON 和 RES-ARG 的血清 Arg 浓度高于 RES（P≤0.03），第 54 天，RES-ARG 高于 CON（P=0.002），RES 处于中间值，与 CON 和 RES-ARG 无差异（P≥0.09）。与 CON 相比，仔羊血清 Orn 和 Cit 较低（P≤0.03）。结果表明，由于母羊的 RES，远侧组织血液灌注减少，而 RES-ARG 能够改善灌注，但不能达到 CON 母羊的水平。此外，母体 RP-Arg 改变了产后期间后代 Arg 和相关氨基酸的浓度。
