Uribe A, Tribukait B, Johansson C
Scand J Gastroenterol. 1987 Mar;22(2):177-84. doi: 10.3109/00365528708991877.
Proliferative and functional epithelial cells were isolated from jejunal specimens of the rat by means of vibrational treatment combined with differential air insufflation. This method gave a good separation between superficial cells of the villi and the crypt cells, as evaluated by flow cytometry, morphology, cytology, and incorporation of radioactive thymidine into DNA. Groups of Sprague-Dawley rats (260 g) were treated twice daily for 11 days with oral placebo, 15(R)15-methyl-prostaglandin E2 in the range of 0.125-2 mg X kg-1, or 5 mg X kg-1 natural PGE2. Isolated crypt cells and superficial cells of the jejunal villi were then analysed by flow cytometry. Morphometric measurements were performed on sections of some jejunal specimens not submitted to vibrational treatment. The cell cycle distribution of crypt cells was unaffected by treatment with the prostaglandin analogue despite the presence of trophic changes. The proportion of crypt cells in G2/M phase was slightly but significantly reduced in rats given natural PGE2 compared with controls. The cell cycle distribution of villus cells was not affected by prostaglandin treatment. Trophic changes in the absence of increased DNA synthesis (S phase) or increased mitotic activity suggests that the hyperplasia observed after prostaglandin treatment is due to a reduced cell loss and/or slower migration time of epithelial cells.
通过振动处理结合差异充气的方法，从大鼠空肠标本中分离出增殖性和功能性上皮细胞。通过流式细胞术、形态学、细胞学以及将放射性胸苷掺入DNA等方法评估，该方法能很好地分离绒毛表面细胞和隐窝细胞。将Sprague-Dawley大鼠（260克）分组，每天口服安慰剂、0.125 - 2毫克/千克体重的15(R)15-甲基前列腺素E2或5毫克/千克体重的天然PGE2，每日两次，持续11天。然后通过流式细胞术分析空肠绒毛的分离隐窝细胞和表面细胞。对一些未进行振动处理的空肠标本切片进行形态测量。尽管存在营养变化，但前列腺素类似物处理对隐窝细胞的细胞周期分布没有影响。与对照组相比，给予天然PGE2的大鼠中G2/M期隐窝细胞的比例略有但显著降低。前列腺素处理对绒毛细胞的细胞周期分布没有影响。在没有DNA合成增加（S期）或有丝分裂活性增加的情况下出现的营养变化表明，前列腺素处理后观察到的增生是由于上皮细胞的细胞损失减少和/或迁移时间减慢所致。
