单独使用噬菌体鸡尾酒和联合使用达托霉素对抗达托霉素不敏感粪肠球菌的评估。
Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium.
机构信息
Anti-Infective Research Laboratory, Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, Michigan, USA.
Department of Infectious Diseases, University of Colorado School of Medicine, Aurora, Colorado, USA.
出版信息
Antimicrob Agents Chemother. 2022 Jan 18;66(1):e0162321. doi: 10.1128/AAC.01623-21. Epub 2021 Nov 1.
Abstract
Enterococcus faecium is a significant multidrug-resistant pathogen. Bacteriophage cocktails are being proposed to complement antibiotic therapy. After a screen of 8 E. faecium strains against 4 phages, 2 phages (113 and 9184) with the broadest host ranges were chosen for further experiments. Transmission electron microscopy, whole-genome sequencing, comparative genome analyses, and time-kill analyses were performed. Daptomycin (DAP) plus the phage cocktail (113 [myophage] and 9184 [siphopage]) showed bactericidal activity in most regimens, while DAP addition prevented phage 9184 resistance against daptomycin-nonsusceptible E. faecium.
摘要
屎肠球菌是一种重要的多药耐药病原体。噬菌体鸡尾酒被提议作为抗生素治疗的补充。在对 8 株屎肠球菌对抗 4 种噬菌体进行筛选后，选择了具有最广泛宿主范围的 2 种噬菌体（113 和 9184）进行进一步实验。进行了透射电子显微镜检查、全基因组测序、比较基因组分析和时间杀伤分析。在大多数方案中，万古霉素（DAP）加噬菌体鸡尾酒（113[myophage]和 9184[siphopage]）显示出杀菌活性，而 DAP 的添加阻止了对万古霉素不敏感的屎肠球菌中噬菌体 9184 对万古霉素的耐药性。
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