噬菌体-抗生素联合策略：对抗耐甲氧西林金黄色葡萄球菌表型的一种替代方法
Bacteriophage-Antibiotic Combination Strategy: an Alternative against Methicillin-Resistant Phenotypes of Staphylococcus aureus.
作者信息
Kebriaei Razieh, Lev Katherine, Morrisette Taylor, Stamper Kyle C, Abdul-Mutakabbir Jacinda C, Lehman Susan M, Morales Sandra, Rybak Michael J
机构信息
Anti-Infective Research Laboratory, College of Pharmacy and Health Sciences, Wayne State University, Detroit, Michigan, USA.
Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA.
出版信息
Antimicrob Agents Chemother. 2020 Jun 23;64(7). doi: 10.1128/AAC.00461-20.
Abstract
Comparative time-kill experiments with bacteriophage (phage) Sb-1 alone and phage-antibiotic combinations (PACs) against two methicillin-resistant (MRSA) strains have shown synergy with both daptomycin-phage and vancomycin-phage combinations. PACs prevented development of phage resistance and demonstrated bactericidal activity for all triple combinations. In addition, the extracellular membrane vesicle (MV) formation and the potential impact of phage on MV suppression were examined. Our results demonstrate the potential of PAC for combating MRSA infections.
摘要
单独使用噬菌体（phage）Sb-1以及噬菌体 - 抗生素组合（PACs）针对两种耐甲氧西林金黄色葡萄球菌（MRSA）菌株进行的比较时间杀菌实验表明，达托霉素 - 噬菌体和万古霉素 - 噬菌体组合均具有协同作用。PACs可防止噬菌体抗性的产生，并对所有三联组合均显示出杀菌活性。此外，还研究了细胞外膜泡（MV）的形成以及噬菌体对MV抑制的潜在影响。我们的结果证明了PACs在对抗MRSA感染方面的潜力。
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